Puerta M, Rocha M, González-Covaleda S, McBennett S M, Andrews J F
Department of Animal Biology II (Physiology), Faculty of Biological Sciences, Complutense University, Madrid, Spain.
Eur J Endocrinol. 1998 Oct;139(4):433-7. doi: 10.1530/eje.0.1390433.
Cold-acclimated female Wistar rats were killed at a known phase of the oestrous cycle to investigate whether natural fluctuations of female sex steroids during the cycle are mirrored by changes in brown adipose tissue (BAT) activity. Four phases: proestrus, oestrus, dioestrus I and dioestrus II, were identified by the cells types appearing in vaginal smears. BAT was removed and analysed for composition and specific indicators of BAT activity. No changes in BAT mass, protein or mitochondrial content were detected during the cycle. Thermogenesis was also unchanged as judged by both lipid multilocularity and mitochondrial GDP-binding. However, cytochrome c oxidase (COX) activity did cycle with a minimum at proestrus and a maximum at dioestrus I. The involvement of oestradiol in such a cycle is suggested by the fact that oestradiol treatment decreased COX activity to values similar to those found in proestrus. The physiological function of a cyclic oxidative capacity remains to be elucidated.
将处于已知动情周期阶段的冷适应雌性Wistar大鼠处死，以研究雌性性激素在周期中的自然波动是否反映在棕色脂肪组织（BAT）活性的变化上。通过阴道涂片出现的细胞类型确定了四个阶段：动情前期、动情期、间情期I和间情期II。取出BAT并分析其组成和BAT活性的特定指标。在整个周期中未检测到BAT质量、蛋白质或线粒体含量的变化。根据脂质多房性和线粒体GDP结合判断，产热也未发生变化。然而，细胞色素c氧化酶（COX）活性确实呈周期性变化，在动情前期最低，在间情期I最高。雌二醇参与这样一个周期的情况是由以下事实表明的：雌二醇处理使COX活性降低至与动情前期相似的值。周期性氧化能力的生理功能仍有待阐明。
