Avila C P, Bartsch D U, Bitner D G, Cheng L, Mueller A J, Karavellas M P, Freeman W R
Department of Ophthalmology, Shiley Eye Center, University of California San Diego, La Jolla 92093-0946, USA.
Am J Ophthalmol. 1998 Nov;126(5):683-90. doi: 10.1016/s0002-9394(98)00114-7.
To determine capillary blood flow measurements in eyes with branch retinal vein occlusion using a scanning laser Doppler flowmeter.
Retinal capillary blood flow in branch retinal vein occlusion areas and corresponding ipsilateral nonbranch retinal vein occlusion areas, 11 equivalent areas of the contralateral fellow eye of 12 consecutive untreated branch retinal vein occlusion patients, and 16 eyes of 11 age-matched normal control subjects were measured with scanning laser Doppler flowmetry. A template consisting of eight squares, each with a field of 100 x 100 microm (10 x 10 pixel) with space interval of 500 microm equidistant horizontally and vertically was used to obtain blood flow measurements in all subjects. Mean blood volume, flow, and velocity were obtained by averaging the mean values measured in each field. We avoided measurement over large retinal vessels to prevent the aliasing artifact of blood cells from moving faster than the sampling frequency.
Branch retinal vein occlusion areas have significantly decreased microvascular blood volume (P = .0009), flow (P = .02), and velocity (P = .016) compared with ipsilateral nonbranch retinal vein occlusion areas in the same eye. Branch retinal vein occlusion areas also have decreased blood volume (P = .001), flow (P = .0042), and velocity (P = .0044) compared with areas of contralateral fellow eyes of branch retinal vein occlusion subjects. Branch retinal vein occlusion areas have significantly decreased blood volume (P = .0012), flow (P = .008), and velocity (P = .02) compared with age-matched normal areas.
Average retinal blood volume, flow, and velocity in areas of branch retinal vein occlusion are significantly lower than in healthy retinas. The ability to noninvasively measure hemodynamic changes in the retinal capillary bed may be relevant to development of new therapies for retinovascular disease.
使用扫描激光多普勒血流仪测定视网膜分支静脉阻塞患者眼部的毛细血管血流情况。
采用扫描激光多普勒血流仪测量12例未经治疗的连续视网膜分支静脉阻塞患者视网膜分支静脉阻塞区域及同侧非分支视网膜静脉阻塞区域、对侧眼11个等效区域以及11名年龄匹配的正常对照者的16只眼睛的视网膜毛细血管血流。使用由8个正方形组成的模板，每个正方形的视野为100×100微米（10×10像素），水平和垂直方向的间隔均为500微米，以获取所有受试者的血流测量值。通过对每个视野测量的平均值进行平均来获得平均血容量、血流量和血流速度。我们避免在大的视网膜血管上进行测量，以防止血细胞移动速度超过采样频率而产生混叠伪像。
与同一只眼睛的同侧非分支视网膜静脉阻塞区域相比，视网膜分支静脉阻塞区域的微血管血容量（P = 0.0009）、血流量（P = 0.02）和血流速度（P = 0.016）显著降低。与视网膜分支静脉阻塞患者对侧眼的区域相比，视网膜分支静脉阻塞区域的血容量（P = 0.001）、血流量（P = 0.0042）和血流速度（P = 0.0044）也降低。与年龄匹配的正常区域相比，视网膜分支静脉阻塞区域的血容量（P = 0.0012）、血流量（P = 0.008）和血流速度（P = 0.02）显著降低。
视网膜分支静脉阻塞区域的平均视网膜血容量、血流量和血流速度显著低于健康视网膜。无创测量视网膜毛细血管床血流动力学变化的能力可能与视网膜血管疾病新疗法的开发有关。
