用于检测临床样本中单纯疱疹病毒的直接免疫荧光细胞离心涂片法的评估。

Evaluation of a direct immunofluorescence cytospin assay for the detection of herpes simplex virus in clinical samples.

作者信息

Reina J, Saurina J, Fernandez-Baca V, Munar M, Blanco I

机构信息

Virology Unit, Clinical Microbiology Service, University Hospital Son Dureta (UIB), Palma de Mallorca, Spain.

出版信息

Eur J Clin Microbiol Infect Dis. 1997 Nov;16(11):851-4. doi: 10.1007/BF01700419.

DOI:10.1007/BF01700419

PMID:9447911

Abstract

A comparison between a direct immunofluorescence assay (DFA) and the shell-vial culture (SVC) was conducted to evaluate their efficacies according to the quality and origin of the sample and the type of herpes simplex (HSV) responsible for the infection. The SVC detected all 58 HSV-infected samples, while the DFA detected only 49 (84.5%) positive samples. The DFA detected HSV type 1 in 22 of 89 samples (24.7%) and HSV type 2 in 27 of 96 samples (28.1%). Compared with the SVC, the DFA had a sensitivity of 75.8% for HSV type 1 and 93.1% for HSV type 2. The sensitivity of the DFA depends on the quality of the sample. Thus, while DFA is recommendable as a screening method, the SVC remains the method of choice for obtaining the maximum diagnostic yield from the sample.

摘要

进行了直接免疫荧光测定法（DFA）和空斑试验培养法（SVC）之间的比较，以根据样本的质量和来源以及引起感染的单纯疱疹病毒（HSV）类型评估它们的效力。SVC检测出所有58份HSV感染样本，而DFA仅检测出49份（84.5%）阳性样本。DFA在89份样本中的22份（24.7%）中检测出1型HSV，在96份样本中的27份（28.1%）中检测出2型HSV。与SVC相比，DFA对1型HSV的敏感性为75.8%，对2型HSV的敏感性为93.1%。DFA的敏感性取决于样本质量。因此，虽然DFA作为一种筛查方法是值得推荐的，但SVC仍然是从样本中获得最大诊断率的首选方法。