See D M, Cimoch P, Chou S, Chang J, Tilles J
University of California, Irvine, Department of Medicine, Orange 92668, USA.
Integr Physiol Behav Sci. 1998 Jul-Sep;33(3):280-7. doi: 10.1007/BF02688668.
In humans, eight monosaccharides are required for the synthesis of glycoproteins. Dietary supplements that supply these crucial sugars are known as glyconutrients. A glyconutrient compound was added to Peripheral Blood Mononuclear Cells (PBMC) isolated from normal controls and patients with the Chronic Fatigue Syndrome (CFS), a disease associated with immune dysregulation. The in vitro immunomodulatory effects were investigated. Cell surface expression of the glycoproteins CD5, CD8, and CD11a were significantly lower in patients with CFS compared to normal controls. Addition of glyconutrient homogenate to PBMC from patients with CFS stimulated with phytohemagglutinin significantly increased the expression of each glycoprotein. Furthermore, natural killer (NK) cell function was reduced in CFS patients. The glyconutrient preparation significantly enhanced NK cell activity versus human herpes virus 6 (HHV-6)-infected H9 cells in an 8 h 51Cr release assay compared to placebo for PBMC from patients with CFS (p< .01). Finally, apoptosis was significantly higher in patients with CFS. The percentage of apoptotic cells was significantly decreased in PBMC from patients with CFS that had been incubated for 48 h with glyconutrients. Thus, glyconutrients improved abnormal immune parameters in vitro in patients with CFS.
在人类中，合成糖蛋白需要八种单糖。提供这些关键糖类的膳食补充剂被称为糖营养素。将一种糖营养素化合物添加到从正常对照者和慢性疲劳综合征（CFS）患者中分离出的外周血单核细胞（PBMC）中，CFS是一种与免疫失调相关的疾病。研究了其体外免疫调节作用。与正常对照者相比，CFS患者糖蛋白CD5、CD8和CD11a的细胞表面表达显著降低。将糖营养素匀浆添加到用植物血凝素刺激的CFS患者的PBMC中，可显著增加每种糖蛋白的表达。此外，CFS患者的自然杀伤（NK）细胞功能降低。在一项8小时51Cr释放试验中，与CFS患者PBMC的安慰剂相比，糖营养素制剂显著增强了对人疱疹病毒6（HHV-6）感染的H9细胞的NK细胞活性（p<0.01）。最后，CFS患者的细胞凋亡显著更高。用糖营养素孵育48小时的CFS患者的PBMC中，凋亡细胞的百分比显著降低。因此，糖营养素在体外改善了CFS患者异常的免疫参数。
