通过免疫荧光显微镜技术对大肠杆菌分裂和非分裂细胞中细胞分裂蛋白FtsQ进行定位。
Localization of cell division protein FtsQ by immunofluorescence microscopy in dividing and nondividing cells of Escherichia coli.
作者信息
Buddelmeijer N, Aarsman M E, Kolk A H, Vicente M, Nanninga N
机构信息
Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, 1098 SM Amsterdam, The Netherlands.
出版信息
J Bacteriol. 1998 Dec;180(23):6107-16. doi: 10.1128/JB.180.23.6107-6116.1998.
Abstract
The localization of cell division protein FtsQ in Escherichia coli wild-type cells was studied by immunofluorescence microscopy with specific monoclonal antibodies. FtsQ could be localized to the division site in constricting cells. FtsQ could also localize to the division site in ftsQ1(Ts) cells grown at the permissive temperature. A hybrid protein in which the cytoplasmic domain and the transmembrane domain were derived from the gamma form of penicillin-binding protein 1B and the periplasmic domain was derived from FtsQ was also able to localize to the division site. This result indicates that the periplasmic domain of FtsQ determines the localization of FtsQ, as has also been concluded by others for the periplasmic domain of FtsN. Noncentral FtsQ foci were found in the area of the cell where the nucleoid resides and were therefore assumed to represent sites where the FtsQ protein is synthesized and simultaneously inserted into the cytoplasmic membrane.
摘要
利用特异性单克隆抗体，通过免疫荧光显微镜技术研究了细胞分裂蛋白FtsQ在大肠杆菌野生型细胞中的定位。FtsQ可定位于正在缢缩细胞的分裂位点。在允许温度下生长的ftsQ1(Ts)细胞中，FtsQ也可定位于分裂位点。一种杂合蛋白，其胞质结构域和跨膜结构域源自青霉素结合蛋白1B的γ形式，周质结构域源自FtsQ，该杂合蛋白也能够定位于分裂位点。这一结果表明，FtsQ的周质结构域决定了FtsQ的定位，其他人对FtsN的周质结构域也得出了同样的结论。在类核所在的细胞区域发现了非中央FtsQ焦点，因此推测这些焦点代表FtsQ蛋白合成并同时插入细胞质膜的位点。
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本文引用的文献
1
FtsI and FtsW are localized to the septum in Escherichia coli.FtsI和FtsW定位于大肠杆菌的隔膜处。
J Bacteriol. 1998 Jun;180(11):2810-6. doi: 10.1128/JB.180.11.2810-2816.1998.
2
The Escherichia coli SRP and SecB targeting pathways converge at the translocon.大肠杆菌的信号识别颗粒（SRP）和SecB靶向途径在转运体处交汇。
EMBO J. 1998 May 1;17(9):2504-12. doi: 10.1093/emboj/17.9.2504.
3
FtsZ dynamics during the division cycle of live Escherichia coli cells.活的大肠杆菌细胞分裂周期中的FtsZ动态变化。
J Bacteriol. 1998 Apr;180(8):2050-6. doi: 10.1128/JB.180.8.2050-2056.1998.
4
Morphogenesis of Escherichia coli.大肠杆菌的形态发生
Microbiol Mol Biol Rev. 1998 Mar;62(1):110-29. doi: 10.1128/MMBR.62.1.110-129.1998.
5
Localization of cell division protein FtsK to the Escherichia coli septum and identification of a potential N-terminal targeting domain.细胞分裂蛋白FtsK在大肠杆菌隔膜上的定位以及一个潜在的N端靶向结构域的鉴定。
J Bacteriol. 1998 Mar;180(5):1296-304. doi: 10.1128/JB.180.5.1296-1304.1998.
6
The MinE ring: an FtsZ-independent cell structure required for selection of the correct division site in E. coli.MinE环：一种大肠杆菌中选择正确分裂位点所需的不依赖FtsZ的细胞结构。
Cell. 1997 Nov 28;91(5):685-94. doi: 10.1016/s0092-8674(00)80455-9.
7
Localization of the Escherichia coli cell division protein Ftsl (PBP3) to the division site and cell pole.大肠杆菌细胞分裂蛋白Ftsl（PBP3）在分裂位点和细胞极的定位。
Mol Microbiol. 1997 Aug;25(4):671-81. doi: 10.1046/j.1365-2958.1997.5041869.x.
8
FtsN, a late recruit to the septum in Escherichia coli.FtsN，一种在大肠杆菌中较晚定位于隔膜的蛋白。
Mol Microbiol. 1997 Jul;25(2):303-9. doi: 10.1046/j.1365-2958.1997.4641833.x.
9
The membrane-bound cell division protein DivIB is localized to the division site in Bacillus subtilis.膜结合细胞分裂蛋白DivIB定位于枯草芽孢杆菌的分裂位点。
Mol Microbiol. 1997 Jul;25(2):275-83. doi: 10.1046/j.1365-2958.1997.4581822.x.
10
ftsW is an essential cell-division gene in Escherichia coli.ftsW是大肠杆菌中的一个必需细胞分裂基因。
Mol Microbiol. 1997 Jun;24(6):1263-73. doi: 10.1046/j.1365-2958.1997.4091773.x.
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