Yu X C, Weihe E K, Margolin W
Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas 77030, USA.
J Bacteriol. 1998 Dec;180(23):6424-8. doi: 10.1128/JB.180.23.6424-6428.1998.
FtsK is essential for Escherichia coli cell division. We report that cells lacking the C terminus of FtsK are defective in chromosome segregation as well as septation, often exhibiting asymmetrically positioned nucleoids and large anucleate regions. Combining the corresponding truncated ftsK gene with a mukB null mutation resulted in a synthetic lethal phenotype. When the truncated ftsK was combined with a minCDE deletion, chains of minicells were generated, many of which contained DNA. These results suggest that the C terminus of FtsK has an important role in chromosome partitioning.
FtsK对大肠杆菌的细胞分裂至关重要。我们报告称，缺乏FtsK C末端的细胞在染色体分离以及隔膜形成方面存在缺陷，常常表现出类核定位不对称和大的无核区域。将相应的截短ftsK基因与mukB无效突变相结合会导致合成致死表型。当截短的FtsK与minCDE缺失相结合时，会产生微细胞链，其中许多含有DNA。这些结果表明，FtsK的C末端在染色体分配中具有重要作用。
