Stefanidou M, Pappas F, Methenitou G, Dona A, Alevisopoulos G, Koutselinis A
Department of Forensic Medicine and Toxicology, University of Athens, Medical School, Greece.
Vet Hum Toxicol. 1998 Dec;40(6):326-7.
The inhibition of pseudocholinesterase (butyrylcholinesterase) activity is a valid and sensitive biochemical indicator of exposure to anticholinesterase insecticides and is often mandatory of the agricultural use of these pesticides. In the present work we developed a modification of an easy and sensitive technique to detect the pseudocholinesterase inhibition due to pesticide exposure. Small amounts of head tissue are needed and low concentrations of the enzyme can be detected. The determination of pseudocholinesterase inhibition is a useful biomonitor of anticholinesterase pesticide exposure for bees in the same way that it is useful for the determination of exposure in humans.
假性胆碱酯酶（丁酰胆碱酯酶）活性的抑制是接触抗胆碱酯酶杀虫剂的一个有效且敏感的生化指标，并且在这些农药的农业使用中通常是必需的。在本研究中，我们对一种简便灵敏的技术进行了改进，以检测因接触农药导致的假性胆碱酯酶抑制。该方法所需的头部组织量少，并且能够检测低浓度的酶。假性胆碱酯酶抑制的测定对于蜜蜂接触抗胆碱酯酶农药而言是一种有用的生物监测指标，其作用方式与对人类接触情况的测定相同。
