Agarwal S, Rao A V
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Ontario, Canada.
Lipids. 1998 Oct;33(10):981-4. doi: 10.1007/s11745-998-0295-6.
Increase in low density lipoprotein (LDL) oxidation is hypothesized to be causally associated with increasing risk of atherosclerosis and coronary heart disease. In recent epidemiological studies, tissue and serum levels of lycopene, a carotenoid available from tomatoes, have been found to be inversely related to risk of coronary heart disease. A study was undertaken to investigate the effect of dietary supplementation of lycopene on LDL oxidation in 19 healthy human subjects. Dietary lycopene was provided using tomato juice, spaghetti sauce, and tomato oleoresin for a period of 1 wk each. Blood samples were collected at the end of each treatment. Serum lycopene was extracted and measured by high-performance liquid chromatography using an absorbance detector. Serum LDL was isolated by precipitation with buffered heparin, and thiobarbituric acid-reactive substances (TBARS) and conjugated dienes (CD) were measured to estimate LDL oxidation. Both methods, to measure LDL oxidation LDL-TBARS and LDL-CD, were in good agreement with each other. Dietary supplementation of lycopene significantly increased serum lycopene levels by at least twofold. Although there was no change in serum cholesterol levels (total, LDL, or high-density lipoprotein), serum lipid peroxidation and LDL oxidation were significantly decreased. These results may have relevance for decreasing the risk for coronary heart disease.
低密度脂蛋白（LDL）氧化增加被认为与动脉粥样硬化和冠心病风险增加存在因果关系。在最近的流行病学研究中，已发现番茄红素（一种可从番茄中获取的类胡萝卜素）的组织和血清水平与冠心病风险呈负相关。开展了一项研究，以调查膳食补充番茄红素对19名健康人体受试者LDL氧化的影响。分别使用番茄汁、意大利面酱和番茄油树脂提供膳食番茄红素，各为期1周。在每次治疗结束时采集血样。通过使用吸光检测器的高效液相色谱法提取并测量血清番茄红素。通过用缓冲肝素沉淀分离血清LDL，并测量硫代巴比妥酸反应性物质（TBARS）和共轭二烯（CD）以评估LDL氧化。测量LDL氧化的两种方法，即LDL-TBARS和LDL-CD，彼此之间具有良好的一致性。膳食补充番茄红素使血清番茄红素水平显著提高至少两倍。尽管血清胆固醇水平（总胆固醇、LDL或高密度脂蛋白）没有变化，但血清脂质过氧化和LDL氧化显著降低。这些结果可能与降低冠心病风险有关。
