Takahashi T, Kamiya T, Yokoo Y
Tsukuba Research Laboratories, Kyowa Hakko Kogyo Co., Ibaraki, Japan.
Acta Derm Venereol. 1998 Nov;78(6):428-32. doi: 10.1080/000155598442719.
For the purpose of discovering natural products which possess hair growing activity, we examined about 1000 kinds of plant extracts concerning growth-promoting activity with respect to hair follicle cells. After an extensive search, we discovered that proanthocyanidins extracted from grape seeds promote proliferation of hair follicle cells isolated from mice by about 230% relative to controls (100%); and that proanthocyanidins possess remarkable hair-cycle-converting activity from the telogen phase to the anagen phase in C3H mice in vivo test systems. The profile of the active fraction of the proanthocyanidins was elucidated by thiolytic degradation and tannase hydrolysis. We found that the constitutive monomers were epicatechin and catechin; and that the degree of polymerization was 3.5. We demonstrated the possibility of using the proanthocyanidins extracted from grape seeds as agents inducing hair growth.
为了发现具有生发活性的天然产物，我们检测了约1000种植物提取物对毛囊细胞的促生长活性。经过广泛搜索，我们发现从葡萄籽中提取的原花青素可使从小鼠分离出的毛囊细胞的增殖相对于对照组（100%）提高约230%；并且在C3H小鼠体内测试系统中，原花青素具有从休止期到生长期的显著毛发周期转换活性。通过硫解降解和单宁酶水解阐明了原花青素活性成分的概况。我们发现组成单体为表儿茶素和儿茶素；聚合度为3.5。我们证明了使用从葡萄籽中提取的原花青素作为诱导头发生长剂的可能性。
