Wasser S, Ho J M, Ang H K, Tan C E
Department of Pediatric Surgery, KK Women's and Children's Hospital, Singapore, Singapore.
J Hepatol. 1998 Nov;29(5):760-71. doi: 10.1016/s0168-8278(98)80257-2.
BACKGROUND/AIMS: Hepatic fibrosis occurs as a result of injury to the liver parenchyma and biliary system. We have studied the effect of the traditional Chinese medicinal herb, Salvia miltiorrhiza, in an experimental model of hepatic fibrosis and evaluated its effect on various paradigms involved in hepatic fibrosis.
Liver fibrosis was induced in male Wistar rats by chronic administration of carbon tetrachloride for 10 weeks. The carbon tetrachloride-treated rats were randomly assigned to three groups: no treatment, Salvia for 12 weeks from the onset of carbon tetrachloride treatment, and Salvia for 2 weeks after the completion of the 10-week course. The normal control groups in the study were: neither carbon tetrachloride nor Salvia, and Salvia only for 12 weeks. The livers were graded histologically and analyzed by reverse transcription-polymerase chain reaction for the transcription of genes involved in liver fibrosis, namely, transforming growth factor-beta1 and the extracellular matrix components procollagens I and III, tissue inhibitor of metalloproteinase-1 and matrix metalloproteinase-13. The transcripts were normalized against that of glyceraldehyde-3-phosphate dehydrogenase and analyzed statistically.
The histological evaluation showed that Salvia could reverse the fibrosis caused by carbon tetrachloride treatment. Rats treated with the herb had reduced levels of transforming growth factor-beta1, procollagens I and III and tissue inhibitor of metalloproteinase-1 transcripts and an increased level of matrix metalloproteinase-13 transcript, when compared to the disease control.
Salvia miltiorrhiza, a cheap and widely available herb, significantly reduces carbon tetrachloride-induced hepatic fibrosis in rats.
背景/目的：肝纤维化是肝实质和胆道系统损伤的结果。我们在肝纤维化实验模型中研究了传统中药丹参的作用，并评估了其对肝纤维化相关各种指标的影响。
通过对雄性Wistar大鼠连续10周给予四氯化碳诱导肝纤维化。将四氯化碳处理的大鼠随机分为三组：不治疗组；从四氯化碳处理开始给予丹参治疗12周组；在10周疗程结束后给予丹参治疗2周组。研究中的正常对照组为：既不给予四氯化碳也不给予丹参组，以及仅给予丹参12周组。对肝脏进行组织学分级，并通过逆转录-聚合酶链反应分析参与肝纤维化的基因转录情况，即转化生长因子-β1、细胞外基质成分I型和III型前胶原、金属蛋白酶组织抑制剂-1和基质金属蛋白酶-13。将转录本以甘油醛-3-磷酸脱氢酶的转录本进行标准化并进行统计学分析。
组织学评估显示丹参可逆转四氯化碳处理所致的纤维化。与疾病对照组相比，用该草药治疗的大鼠转化生长因子-β1、I型和III型前胶原以及金属蛋白酶组织抑制剂-1转录本水平降低，而基质金属蛋白酶-13转录本水平升高。
丹参作为一种廉价且广泛可得的草药，可显著减轻四氯化碳诱导的大鼠肝纤维化。
