Uvnäs-Wallensten K, Rehfeld J F, Larsson L I, Uvnäs B
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5707-10. doi: 10.1073/pnas.74.12.5707.
Immunoreactive gastrin was present in vagal nerves from cats, dogs, and human beings. The abdominal portion of the vagus contained gastrin in amounts ranging from 16 to 273 pmol/g of nerve tissue (wet weight). The thoracic and cervical portion of the vagi contained only minute amounts of gastrin. Gel chromatography of extracts of human, canine, and feline abdominal vagi monitored by region-specific antisera against heptadecapeptide gastrin and triacontatriapeptide cholecystokinin revealed that the vagal gastrin immunoreactivity predominantly consisted of heptadecapeptide gastrin. In addition, the vagi contained small amounts of the NH2-terminal tridecapeptide gastrin fragment as well as of the putative biosynthetic gastrin precursors, components I and II. No cholecystokinin-like molecules were demonstrable. Immunocytochemical studies demonstrated gastrin-containing nerves in the intestinal wall. The nerves were found to be most numerous in the large and distal small intestine. These findings suggest that heptadecapeptide gastrin may represent a new vagal neurotransmitter.
免疫反应性胃泌素存在于猫、狗和人类的迷走神经中。迷走神经的腹部部分含有胃泌素，含量范围为每克神经组织（湿重）16至273皮摩尔。迷走神经的胸段和颈段仅含有微量的胃泌素。用人、犬和猫腹部迷走神经提取物进行凝胶色谱分析，并用针对十七肽胃泌素和三十三肽胆囊收缩素的区域特异性抗血清监测，结果显示迷走神经胃泌素免疫反应性主要由十七肽胃泌素组成。此外，迷走神经还含有少量的氨基末端十三肽胃泌素片段以及假定的生物合成胃泌素前体成分I和II。未检测到胆囊收缩素样分子。免疫细胞化学研究表明肠壁中有含胃泌素的神经。发现这些神经在大肠和小肠远端最多。这些发现表明十七肽胃泌素可能代表一种新的迷走神经递质。
