Alicea C, Belkowski S M, Sliker J K, Zhu J, Liu-Chen L Y, Eisenstein T K, Adler M W, Rogers T J
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
J Neuroimmunol. 1998 Nov 2;91(1-2):55-62. doi: 10.1016/s0165-5728(98)00151-9.
We have found that the immature T cell lines R1.1 and DPK and the macrophage lines P388D1 and WEHI-3 also express kappa-opioid receptor (KOR) mRNA. Characterization of the KOR transcripts in both brain tissue and these T cells has revealed both the normal full-length as well as a truncated form of the mRNA. Our results show that the truncated transcript lacks the second exon. Primary macrophages express this truncated form of the transcript in the absence of detectable levels of the full-length form. These results suggest a degree of heterogeneity in the expression of the opioid receptors which has not previously been reported.
我们发现未成熟T细胞系R1.1和DPK以及巨噬细胞系P388D1和WEHI-3也表达κ-阿片受体（KOR）mRNA。对脑组织和这些T细胞中KOR转录本的表征揭示了正常的全长mRNA以及截短形式的mRNA。我们的结果表明，截短的转录本缺少第二个外显子。原代巨噬细胞在未检测到全长形式的情况下表达这种截短形式的转录本。这些结果表明阿片受体表达存在一定程度的异质性，这在以前尚未见报道。
