Fleischmajer R, Perlish J S, Shaw K V, Pirozzi D J
Arch Dermatol. 1976 Nov;112(11):1553-7.
Skin biopsy specimens obtained from involved and noninvolved areas in a patient with early diffuse systemic scleroderma were processed for histology, electron microscopy, and "in vitro" autoradiography with tritiated thymidine. The affected area revealed cellular infiltrates around the eccrine sweat glands, consisting of plasma cells and lymphocytes. The capillaries showed thickening of the basement lamina, damage of endothelial cells, and obstruction of their lumens. However, in some vessels, endothelial cells were preserved and appeared in prophase. Autoradiography with tritiated thymidine showed a marked increase in endothelial and periendothelial cell labeling. Blood immunological studies revealed an increase in B-lymphocytes, IgG, and IgA and the presence of antinuclear and antismooth muscle antibodies.
从一名早期弥漫性系统性硬皮病患者的受累及未受累区域获取皮肤活检标本，进行组织学、电子显微镜检查以及用氚标记胸腺嘧啶核苷进行“体外”放射自显影。受累区域显示在小汗腺周围有细胞浸润，由浆细胞和淋巴细胞组成。毛细血管显示基底膜增厚、内皮细胞损伤及其管腔阻塞。然而，在一些血管中，内皮细胞得以保留并处于前期。用氚标记胸腺嘧啶核苷进行放射自显影显示内皮细胞和内皮周细胞标记显著增加。血液免疫学研究显示B淋巴细胞、IgG和IgA增加，以及存在抗核抗体和抗平滑肌抗体。
