小鼠P2X7受体的克隆与功能特性研究

Cloning and functional characterisation of the mouse P2X7 receptor.

作者信息

Chessell I P, Simon J, Hibell A D, Michel A D, Barnard E A, Humphrey P P

机构信息

Glaxo Institute of Applied Pharmacology, Department of Pharmacology, University of Cambridge, UK.

出版信息

FEBS Lett. 1998 Nov 13;439(1-2):26-30. doi: 10.1016/s0014-5793(98)01332-5.

DOI:10.1016/s0014-5793(98)01332-5

PMID:9849870

Abstract

We have isolated a 1785-bp complementary DNA (cDNA) encoding the murine P2X7 receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80%, and 85% homology to the human and rat P2X7 subunits, respectively. Functional properties of the heterologously expressed murine P2X7 homomeric receptor broadly resembled those of the P2X7 receptor in the native cell line. However, marked phenotypic differences were observed between the mouse receptor, and the other P2X7 receptor orthologues isolated with respect to agonist and antagonist potencies, and the kinetics of formation of the large aqueous pore.

摘要

我们从NTW8小鼠小胶质细胞中分离出了一段1785碱基对的互补DNA（cDNA），其编码小鼠P2X7受体亚基。该编码蛋白与人及大鼠的P2X7亚基分别具有80%和85%的同源性。异源表达的小鼠P2X7同聚体受体的功能特性与天然细胞系中的P2X7受体大致相似。然而，在小鼠受体与其他已分离的P2X7受体直向同源物之间，在激动剂和拮抗剂效力以及大水性孔形成动力学方面观察到了明显的表型差异。