Vermes L M, Ferri R G, Aisen J, Marlet J M
Arq Neuropsiquiatr. 1976 Dec;34(4):315-24. doi: 10.1590/s0004-282x1976000400001.
The total protein content of CSF collected from the cisterna magna (cisternal fluid) of 98 patients (58 males and 40 females) with no neurological diseases was determined by the colorimetric method devised by Lowry and co-workers. This method has been previously chosen based mainly on it's sensitivity, accuracy and low fluid consumption. The CSF samples were normal with regard to pressure, color, citology and it's content in glucose, chloride and urea. Complement fixation tests for syphilis and cisticercosis, as well as the globulin tests (Pandy, Nonne--Appelt and Takata-Ara) were negative. The average value and the normal range of cisternal fluid total protein was calculated for the mixed population (males and females). The mean protein value was 26.78 mg/100 ml, the lower and upper limits were respectively 13.20 and 40.36 mg/100 ml. These data are higher than those stated in some publications, and factors that could interfere in the different results mentioned in the literature are briefly discussed. Since the statistical analysis of the results showed that the mean total protein concentration in males (28.76 mg/100 ml) was higher than in females (23.91 mg/100 ml), normal limits for each sex were established: 16.96 to 47.13 mg/100 ml for males and 14.76 to 42.76 mg/100 ml for females.
采用Lowry及其同事设计的比色法，测定了98例无神经系统疾病患者（58例男性，40例女性）枕大池脑脊液（池液）中的总蛋白含量。此前选择该方法主要是基于其灵敏度、准确性和低耗液量。脑脊液样本在压力、颜色、细胞学以及葡萄糖、氯化物和尿素含量方面均正常。梅毒和囊尾蚴病的补体结合试验以及球蛋白试验（潘迪试验、诺内-阿佩尔特试验和高田-荒试验）均为阴性。计算了混合人群（男性和女性）池液总蛋白的平均值和正常范围。平均蛋白值为26.78mg/100ml，下限和上限分别为13.20mg/100ml和40.36mg/100ml。这些数据高于一些出版物中所述的数据，并简要讨论了可能干扰文献中提及的不同结果的因素。由于结果的统计分析表明男性的平均总蛋白浓度（28.76mg/100ml）高于女性（23.91mg/100ml），因此确定了各性别的正常范围：男性为16.96至47.13mg/100ml，女性为14.76至42.76mg/100ml。
