铜绿假单胞菌的一种新型丝氨酸/苏氨酸蛋白激酶同源物在宿主感染部位特异性诱导表达，并且是中性粒细胞减少小鼠完全致病性所必需的。
A novel serine/threonine protein kinase homologue of Pseudomonas aeruginosa is specifically inducible within the host infection site and is required for full virulence in neutropenic mice.
作者信息
Wang J, Li C, Yang H, Mushegian A, Jin S
机构信息
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA.
出版信息
J Bacteriol. 1998 Dec;180(24):6764-8. doi: 10.1128/JB.180.24.6764-6768.1998.
Abstract
A genetic locus of Pseudomonas aeruginosa was identified that is highly and specifically inducible during infection of neutropenic mice. This locus, ppkA, encodes a protein that is highly homologous to eukaryote-type serine/threonine protein kinases. A ppkA null mutant strain shows reduced virulence in neutropenic mice compared to the wild type. Overexpression of the PpkA protein greatly inhibited the growth of Escherichia coli or P. aeruginosa. However, a single amino acid change at the catalytic site of the kinase domain eliminated the toxic effect of PpkA on bacterial cells, suggesting that the kinase domain of PpkA is functional within bacterial cells.
摘要
鉴定出了铜绿假单胞菌的一个基因座，该基因座在中性粒细胞减少小鼠感染期间具有高度特异性的诱导性。这个基因座，即ppkA，编码一种与真核生物型丝氨酸/苏氨酸蛋白激酶高度同源的蛋白质。与野生型相比，ppkA基因敲除突变株在中性粒细胞减少小鼠中的毒力降低。PpkA蛋白的过表达极大地抑制了大肠杆菌或铜绿假单胞菌的生长。然而，激酶结构域催化位点的单个氨基酸变化消除了PpkA对细菌细胞的毒性作用，这表明PpkA的激酶结构域在细菌细胞内具有功能。
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本文引用的文献
1
Mutants of Escherichia coli requiring methionine or vitamin B12.需要甲硫氨酸或维生素B12的大肠杆菌突变体。
J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
2
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.从全基因组序列解读结核分枝杆菌的生物学特性。
Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
3
Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection.通过体内筛选大规模分离铜绿假单胞菌的候选毒力基因
Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10434-9. doi: 10.1073/pnas.93.19.10434.
4
Analysis of compositionally biased regions in sequence databases.序列数据库中成分偏倚区域的分析。
Methods Enzymol. 1996;266:554-71. doi: 10.1016/s0076-6879(96)66035-2.
5
Homing events in the gyrA gene of some mycobacteria.一些分枝杆菌gyrA基因中的归巢事件。
Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3410-5. doi: 10.1073/pnas.93.8.3410.
6
Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa.铜绿假单胞菌中rpoN基因的核苷酸序列及两个下游开放阅读框的特征分析
J Bacteriol. 1994 Mar;176(5):1316-22. doi: 10.1128/jb.176.5.1316-1322.1994.
7
Cloning, sequencing and expression of serine/threonine kinase-encoding genes from Streptomyces coelicolor A3(2).来自天蓝色链霉菌A3(2)的丝氨酸/苏氨酸激酶编码基因的克隆、测序及表达
Gene. 1995 Feb 3;153(1):99-104. doi: 10.1016/0378-1119(94)00789-u.
8
9
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.蛋白激酶家族：催化结构域的保守特征与推导的系统发育
Science. 1988 Jul 1;241(4861):42-52. doi: 10.1126/science.3291115.
10
Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (RpoN) of RNA polymerase.铜绿假单胞菌中菌毛蛋白的形成需要RNA聚合酶的替代σ因子（RpoN）。
Proc Natl Acad Sci U S A. 1989 Mar;86(6):1954-7. doi: 10.1073/pnas.86.6.1954.
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