Dorigo-Zetsma J W, Zaat S A, Wertheim-van Dillen P M, Spanjaard L, Rijntjes J, van Waveren G, Jensen J S, Angulo A F, Dankert J
Laboratory of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands.
J Clin Microbiol. 1999 Jan;37(1):14-7. doi: 10.1128/JCM.37.1.14-17.1999.
For diagnosis of Mycoplasma pneumoniae infection we compared two rapid tests, PCR and the immunoglobulin M immunofluorescence assay (IgM IFA), with culture and the complement fixation test (CFT), in a prospective study among 92 children with respiratory tract infection and 74 controls. Based on positivity of culture and/or CFT as the diagnostic criterion, nine patients (10%) were diagnosed with M. pneumoniae infection. All patients positive by culture were also positive by PCR. In all controls cultures, PCRs, and serological assays were negative, except in one with a positive IgM IFA. The IgM IFA had a low positive predictive value of 50%. Only a combination of PCR (seven patients) and CFT (seven patients) allowed diagnosis of all cases.
为诊断肺炎支原体感染，我们在一项前瞻性研究中，对92例呼吸道感染儿童和74例对照者，比较了两种快速检测方法（PCR和免疫球蛋白M免疫荧光试验（IgM IFA））与培养及补体结合试验（CFT）的结果。以培养和/或CFT阳性作为诊断标准，9例患者（10%）被诊断为肺炎支原体感染。所有培养阳性的患者PCR检测也呈阳性。在所有对照者中，培养、PCR及血清学检测均为阴性，只有1例IgM IFA呈阳性。IgM IFA的阳性预测值较低，为50%。只有PCR（7例患者）和CFT（7例患者）联合检测才能诊断所有病例。
