Strle F, Cheng Y, Nelson J A, Picken M M, Bouseman J K, Picken R N
Department of Infectious Diseases, University Medical Centre, Ljubljana, Slovenia.
Eur J Clin Microbiol Infect Dis. 1995 Nov;14(11):994-1001. doi: 10.1007/BF01691382.
In spring 1993, Ixodes ricinus ticks were collected from six regions of Slovenia to determine their overall rate of infection with Borrelia burgdorferi sensu lato and to assess the frequency of individual species in these tick populations. Ticks were dissected and midgut tissue inoculated into modified Barbour-Stoenner-Kelly (BSK II) medium. Borrelia isolates were differentiated into separate species using species-specific polymerase chain reaction (PCR) primers and by large restriction fragment pattern (LRFP) analysis. Infected ticks were found in all six regions surveyed. Spirochaetes were isolated from 69 of 363 ticks (19%): the isolation rate from adult female ticks was 35% (23/66 ticks cultured), from adult male ticks 22% (20/91), and from nymphal ticks 13% (26/206). Determination of the species of 60 isolates revealed that 32 were Borrelia afzelii (53%), 20 were Borrelia garinii (33%), and 8 were Borrelia burgdorferi sensu stricto (13%). In the Ljubljana region Borrelia afzelii and Borrelia garinii predominated (43% and 40%, respectively), whereas Borrelia burgdorferi sensu stricto constituted only 17% of isolates. In three other regions of the country Borrelia afzelii was isolated exclusively, although the number of isolates investigated was small. This study demonstrates the presence of all three European species of Borrelia burgdorferi sensu lato within the Slovenian tick population and also within a geographic area of less than 100 m2.
1993年春季，从斯洛文尼亚的六个地区采集了蓖麻硬蜱，以确定其感染广义伯氏疏螺旋体的总体比率，并评估这些蜱虫种群中各个物种的出现频率。将蜱虫解剖，把中肠组织接种到改良的巴伯-斯托纳-凯利（BSK II）培养基中。使用物种特异性聚合酶链反应（PCR）引物并通过大限制性片段模式（LRFP）分析，将伯氏疏螺旋体分离株区分为不同的物种。在所调查的所有六个地区均发现了受感染的蜱虫。从363只蜱虫中的69只（19%）分离出了螺旋体：成年雌蜱的分离率为35%（培养的66只蜱虫中有23只），成年雄蜱为22%（91只中有20只），若蜱为13%（206只中有26只）。对60个分离株进行的物种鉴定显示，32个为阿氏疏螺旋体（53%），20个为伽氏疏螺旋体（33%），8个为狭义伯氏疏螺旋体（13%）。在卢布尔雅那地区，阿氏疏螺旋体和伽氏疏螺旋体占主导地位（分别为43%和40%），而狭义伯氏疏螺旋体仅占分离株的17%。在该国的其他三个地区，仅分离出了阿氏疏螺旋体，不过所研究的分离株数量较少。这项研究证明，在斯洛文尼亚的蜱虫种群中以及在面积小于100平方米的地理区域内均存在广义伯氏疏螺旋体的全部三种欧洲物种。
