用12种混合抗血清通过协同凝集法对肺炎链球菌进行血清分型。
Serotyping of Streptococcus pneumoniae by coagglutination with 12 pooled antisera.
作者信息
Lalitha M K, Thomas K, Kumar R S, Steinhoff M C
机构信息
Department of Clinical Microbiology, Christian Medical College & Hospital, Vellore, India.
出版信息
J Clin Microbiol. 1999 Jan;37(1):263-5. doi: 10.1128/JCM.37.1.263-265.1999.
Abstract
We report on the performance of a recently introduced commercial chessboard method using 12 antisera, in comparison with that of the 55-antiserum panel used in determining the serogroups and types (SGTs) of Streptococcus pneumoniae, both of which were carried out by a coagglutination technique. Of a total of 150 strains of S. pneumoniae studied, 135 (90%) belonged to the SGTs represented in the 23-valent pneumococcal vaccine; of these, 130 (96.3%) were identified as the same SGTs by both typing methods. The remaining five strains showed cross-reactivity with more than two pools by the chessboard method, but could be assigned to a single SGT by the Quellung test. The 96.3% concordance of the chessboard method suggests it can be adopted for determination of the SGTs of S. pneumoniae in laboratories.
摘要
我们报告了一种最近推出的使用12种抗血清的商用棋盘法的性能，并将其与用于确定肺炎链球菌血清群和血清型（SGTs）的55种抗血清板的性能进行了比较，这两种方法均通过协同凝集技术进行。在总共研究的150株肺炎链球菌中，135株（90%）属于23价肺炎球菌疫苗中所包含的血清群和血清型；其中，两种分型方法将130株（96.3%）鉴定为相同的血清群和血清型。其余五株菌株通过棋盘法与两个以上的组合显示出交叉反应，但通过荚膜肿胀试验可归为单一的血清群和血清型。棋盘法96.3%的一致性表明它可在实验室中用于肺炎链球菌血清群和血清型的测定。
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本文引用的文献
1
Modified latex agglutination test for rapid detection of Streptococcus pneumoniae and haemophilus influenzae in cerebrospinal fluid and direct serotyping of Streptococcus pneumoniae.改良乳胶凝集试验用于快速检测脑脊液中的肺炎链球菌和流感嗜血杆菌以及肺炎链球菌的直接血清分型。
Eur J Clin Microbiol Infect Dis. 1996 Jun;15(6):472-7. doi: 10.1007/BF01691314.
2
Serotyping of Streptococcus pneumoniae by agglutination assays: a cost-effective technique for developing countries.通过凝集试验对肺炎链球菌进行血清分型：一种适用于发展中国家的经济高效技术。
Bull World Health Organ. 1996;74(4):387-90.
3
Six newly recognized types of Streptococcus pneumoniae.六种新确认的肺炎链球菌类型。
J Clin Microbiol. 1995 Oct;33(10):2759-62. doi: 10.1128/jcm.33.10.2759-2762.1995.
4
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J Clin Microbiol. 1993 Aug;31(8):2097-100. doi: 10.1128/jcm.31.8.2097-2100.1993.
5
Serotypes of Streptococcus pneumoniae causing meningitis in southern India. Use of new direct latex agglutination antigen detection tests in cerebrospinal fluid.
Diagn Microbiol Infect Dis. 1994 Apr;18(4):211-4. doi: 10.1016/0732-8893(94)90022-1.
6
7
The epidemiology of pneumococcal disease in infants and children.婴幼儿肺炎球菌疾病的流行病学
Rev Infect Dis. 1981 Mar-Apr;3(2):246-53. doi: 10.1093/clinids/3.2.246.
8
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9
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