Constantz B R, Barr B M, Ison I C, Fulmer M T, Baker J, McKinney L, Goodman S B, Gunasekaren S, Delaney D C, Ross J, Poser R D
Norian Corporation, Cupertino, California 95014, USA.
J Biomed Mater Res. 1998 Winter;43(4):451-61. doi: 10.1002/(sici)1097-4636(199824)43:4<451::aid-jbm13>3.0.co;2-q.
Four calcium phosphate cement formulations were implanted in the rabbit distal femoral metaphysis and middiaphysis. Chemical, crystallographic, and histological analyses were made at 2, 4, and 8 weeks after implantation. When implanted into the metaphysis, part of the brushite cement was converted into carbonated apatite by 2 weeks. Some of the brushite cement was removed by mononuclear macrophages prior to its conversion into apatite. Osteoclastlike cell mediated remodeling was predominant at 8 weeks after brushite had converted to apatite. The same histological results were seen for brushite plus calcite aggregate cement, except with calcite aggregates still present at 8 weeks. However, when implanted in the diaphysis, brushite and brushite plus calcite aggregate did not convert to another calcium phosphate phase by 4 weeks. Carbonated apatite cement implanted in the metaphysis did not transform to another calcium phosphate phase. There was no evidence of adverse foreign body reaction. Osteoclastlike cell mediated remodeling was predominant at 8 weeks. The apatite plus calcite aggregate cement implanted in the metaphysis that was not remodeled remained as poorly crystalline apatite. Calcite aggregates were still present at 8 weeks. There was no evidence of foreign body reaction. Osteoclastlike cell remodeling was predominant at 8 weeks. Response to brushite cements prior to conversion to apatite was macrophage dominated, and response to apatite cements was osteoclast dominated. Mineralogy, chemical composition, and osseous implantation site of these calcium phosphates significantly affected their in vivo host response.
将四种磷酸钙骨水泥制剂植入兔股骨远端干骺端和骨干中部。在植入后2周、4周和8周进行化学、晶体学和组织学分析。当植入干骺端时，部分透钙磷石骨水泥在2周时转化为碳酸磷灰石。一些透钙磷石骨水泥在转化为磷灰石之前被单核巨噬细胞清除。透钙磷石转化为磷灰石后8周，破骨细胞样细胞介导的重塑占主导地位。透钙磷石加方解石聚集体骨水泥也观察到相同的组织学结果，只是在8周时仍存在方解石聚集体。然而，当植入骨干时，透钙磷石和透钙磷石加方解石聚集体在4周时未转化为另一种磷酸钙相。植入干骺端的碳酸磷灰石骨水泥未转化为另一种磷酸钙相。没有不良异物反应的证据。8周时破骨细胞样细胞介导的重塑占主导地位。植入干骺端未重塑的磷灰石加方解石聚集体骨水泥仍为结晶不良的磷灰石。8周时仍存在方解石聚集体。没有异物反应的证据。8周时破骨细胞样细胞重塑占主导地位。透钙磷石骨水泥在转化为磷灰石之前以巨噬细胞反应为主，而对磷灰石骨水泥则以破骨细胞反应为主。这些磷酸钙骨水泥的矿物学、化学成分和骨植入部位显著影响其体内宿主反应
