Nadkarni J S, Bhadsavle P R, Chitnis V S, Nadkarni J J, Vyas J S, Desai P B
Division of Laboratory Medicine, Tata Memorial Centre, Bombay, India.
Tumori. 1998 Sep-Oct;84(5):578-82. doi: 10.1177/030089169808400513.
A murine monoclonal antibody (MAb) 2G3 of the IgG1 type was raised using the human esophageal squamous cell carcinoma (SCC) cell line TE-2. Immunoblotting with 2G3 indicated that the antigen recognized by 2G3 has a molecular weight of 34 kD. Its activity was evaluated by immunoperoxidase and immunofluorescence on frozen and paraffin sections of various normal tissues, normal and benign tumors as well as various established cell lines. The pattern of reactivity revealed that the antigen recognized by 2G3 was expressed mainly by esophageal SCC. The only exception was represented by malignant breast tumors, where it reacted weakly. Scatchard analysis using 125I-labelled 2G3 showed that TE-2 has approximately 7.5 times more binding sites than the MCF-7 breast cancer cell line. The use of this new MAb is therefore proposed for the histopathological diagnosis of esophageal SCC.
利用人食管鳞状细胞癌（SCC）细胞系TE-2制备了IgG1型鼠单克隆抗体（MAb）2G3。用2G3进行免疫印迹表明，2G3识别的抗原分子量为34 kD。通过免疫过氧化物酶法和免疫荧光法在各种正常组织、正常及良性肿瘤以及各种已建立的细胞系的冰冻切片和石蜡切片上对其活性进行了评估。反应模式显示，2G3识别的抗原主要在食管鳞状细胞癌中表达。唯一的例外是恶性乳腺肿瘤，在其中反应较弱。使用125I标记的2G3进行的Scatchard分析表明，TE-2的结合位点比MCF-7乳腺癌细胞系多约7.5倍。因此，建议将这种新的单克隆抗体用于食管鳞状细胞癌的组织病理学诊断。
