甲状腺状态对大鼠胰高血糖素受体mRNA的组织特异性调节

Tissue-specific modulation of rat glucagon receptor mRNA by thyroid status.

作者信息

Morales A, Lachuer J, Duchamp C, Vera N, Georges B, Cohen-Adad F, Moulin C, Barré H

机构信息

Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS-UCB LYON 1, Faculté des Sciences, Villeurbanne, France.

出版信息

Mol Cell Endocrinol. 1998 Sep 25;144(1-2):71-81. doi: 10.1016/s0303-7207(98)00150-6.

DOI:10.1016/s0303-7207(98)00150-6

PMID:9863628

Abstract

The influence of thyroid status on glucagon receptor mRNA levels was investigated in rats using a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Glucagon receptor mRNA was detected in liver, brown and white adipose tissues (BAT and WAT) and brain. In BAT and WAT, pharmacologically-induced moderate hypothyroidism resulted in a marked reduction in the relative abundance of glucagon receptor mRNA. Short-term treatment of hypothyroid rats with exogenous 3,3',5'-triiodo-L-thyronine (T3), resulting in a marked hyperthyroidism, reversed the phenomenon in BAT while the reversal was only partial in WAT. In the liver, there was no significant effect of mild hypothyroidism while there was a positive effect of hyperthyroidism. In brain, the relative tissue abundance of glucagon receptor mRNA was not affected by the large changes in plasma T3. The present results therefore indicate that thyroid status may modulate the relative abundance of glucagon receptor mRNA in a tissue-specific manner.

摘要

利用半定量逆转录聚合酶链反应（RT-PCR）分析法，研究了甲状腺状态对大鼠胰高血糖素受体mRNA水平的影响。在肝脏、棕色和白色脂肪组织（BAT和WAT）以及大脑中检测到了胰高血糖素受体mRNA。在BAT和WAT中，药物诱导的中度甲状腺功能减退导致胰高血糖素受体mRNA的相对丰度显著降低。用外源性3,3',5'-三碘-L-甲状腺原氨酸（T3）对甲状腺功能减退大鼠进行短期治疗，导致明显的甲状腺功能亢进，逆转了BAT中的这一现象，而在WAT中仅部分逆转。在肝脏中，轻度甲状腺功能减退没有显著影响，而甲状腺功能亢进有积极影响。在大脑中，血浆T3的大幅变化未影响胰高血糖素受体mRNA的相对组织丰度。因此，目前的结果表明，甲状腺状态可能以组织特异性方式调节胰高血糖素受体mRNA的相对丰度。