Hayashi J, Masaka T, Ishikawa I
Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Infect Immun. 1999 Jan;67(1):417-20. doi: 10.1128/IAI.67.1.417-420.1999.
Soluble CD14 (sCD14) mediates the response to lipopolysaccharide (LPS) in cells lacking membrane-bound CD14. We determined sCD14 concentrations in the sera of 38 periodontitis patients and 25 healthy controls by enzyme-linked immunosorbent assay. The sCD14 levels in the sera of patients with periodontitis were significantly higher than those of healthy subjects and decreased after treatment. Enhanced levels of sCD14 in serum may contribute to the host response to LPS in periodontitis. Furthermore, we showed in vitro that addition of LPS enhanced the release of sCD14 by monoblastic U937 cells treated with 1alpha,25-dihydroxyvitamin D3. Thus, increased sCD14 levels in periodontitis patients may be due to chronic exposure to LPS.
可溶性CD14（sCD14）介导缺乏膜结合型CD14的细胞对脂多糖（LPS）的反应。我们通过酶联免疫吸附测定法测定了38例牙周炎患者和25名健康对照者血清中的sCD14浓度。牙周炎患者血清中的sCD14水平显著高于健康受试者，且治疗后降低。血清中sCD14水平的升高可能有助于宿主对牙周炎中LPS的反应。此外，我们在体外表明，添加LPS可增强经1α,25-二羟基维生素D3处理的单核细胞U937细胞释放sCD14。因此，牙周炎患者sCD14水平升高可能是由于长期接触LPS所致。
