Blasco R, Moore E, Wray V, Pieper D, Timmis K, Castillo F
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, 14071 Córdoba,
J Bacteriol. 1999 Jan;181(1):149-52. doi: 10.1128/JB.181.1.149-152.1999.
The bacterial strain RB1 has been isolated by enrichment cultivation with 2,4-dinitrophenol as the sole nitrogen, carbon, and energy source and characterized, on the basis of 16S rRNA gene sequence comparison, as a Rhodococcus species closely related to Rhodococcus opacus. Rhodococcus sp. strain RB1 degrades 2,4-dinitrophenol, releasing the two nitro groups from the compound as nitrite. The release of nitro groups from 2,4-dinitrophenol occurs in two steps. First, the 2-nitro group is removed as nitrite, with the production of an aliphatic nitro compound identified by 1H nuclear magnetic resonance and mass spectrometry as 3-nitroadipate. Then, this metabolic derivative is further metabolized, releasing its nitro group as nitrite. Full nitrite assimilation upon reduction to ammonia requires that an additional carbon source be supplied to the medium.
细菌菌株RB1是通过以2,4-二硝基苯酚作为唯一氮源、碳源和能源进行富集培养而分离得到的，并根据16S rRNA基因序列比较，鉴定为与不透明红球菌密切相关的红球菌属物种。红球菌属菌株RB1降解2,4-二硝基苯酚，从该化合物中释放出两个硝基作为亚硝酸盐。从2,4-二硝基苯酚中释放硝基分两步进行。首先，2-硝基作为亚硝酸盐被去除，同时产生一种脂肪族硝基化合物，通过1H核磁共振和质谱鉴定为3-硝基己二酸。然后，这种代谢衍生物进一步代谢，释放出其硝基作为亚硝酸盐。还原为氨时完全同化亚硝酸盐需要向培养基中提供额外的碳源。
