Jahn C L, Baran M M, Bachvarova R
J Exp Zool. 1976 Aug;197(2):161-71. doi: 10.1002/jez.1401970202.
The RNA of growing mouse oocytes and ovulated ova was labeled by injection of a solution containing tritiated uridine into the ovarian bursa. The time course of incorporation into RNA by oocytes was followed by analysis of alkali-labile acid insoluble radioactivity and by autoradiography. The results show that most of the incorporation into RNA by growing oocytes takes place within one day of bursal injection of the precursor, reflecting the rapid fall of label in the acid soluble precursor pool. The RNA of growing oocytes of all sizes is unusually stable, at least 80% of the labeled RNA present two days after bursal injection being retained until ovulation 10 to 20 days later. The fraction of heterogeneous RNA in labeled RNA of ova was estimated as 20% by sucrose gradient analysis. It is likely that egg RNA is synthesized primarily during the period of oocyte growth one to three weeks before ovulation.
通过向卵巢囊内注射含有氚标记尿苷的溶液，对生长中的小鼠卵母细胞和排卵后的卵子的RNA进行标记。通过分析碱不稳定酸不溶性放射性和放射自显影来追踪卵母细胞将其掺入RNA的时间进程。结果表明，生长中的卵母细胞将其掺入RNA的过程大部分发生在前体注入卵巢囊后的一天内，这反映了酸溶性前体池中标记物的快速下降。各种大小的生长中卵母细胞的RNA异常稳定，卵巢囊注射两天后存在的标记RNA中至少80%会保留到10至20天后排卵。通过蔗糖梯度分析估计，卵子标记RNA中异质RNA的比例为20%。卵子RNA可能主要在排卵前一至三周的卵母细胞生长期间合成。
