Hoage T R, Cameron I L
Anat Rec. 1976 Dec;186(4):585-94. doi: 10.1002/ar.1091860408.
Mice were injected three times over an 8-hour period with a total of 48 muci 3H-T/gm of body weight and were sacrificed 1, 3, 5, 9 and 17 days afterwards. Radioautographs of the ovaries showed significantly higher grain counts in oocytes of follicles that are in the antrum formation stage. The radioautographic visualization of DNase digestible 3H-thymidine incorporation into the juxtanucleolar region in oocytes of mature mice occurs in association with ooctye growth in follicles that are in the antrum formation stage. The scheduled disappearance of this juxtanucleolar oocyte DNA and its label during later oocyte growth suggests a degradation or dispersion of this labeled DNA prior to ovulation.
在8小时内给小鼠注射三次，总量为每克体重48微居里的³H-T，然后在1、3、5、9和17天后处死小鼠。卵巢的放射自显影片显示，处于卵泡腔形成阶段的卵泡卵母细胞中的颗粒计数明显更高。在成熟小鼠卵母细胞中，³H-胸腺嘧啶核苷掺入核仁旁区域的放射自显影可视化与处于卵泡腔形成阶段的卵泡中卵母细胞的生长相关。在随后的卵母细胞生长过程中，这种核仁旁卵母细胞DNA及其标记物的预定消失表明这种标记的DNA在排卵前发生了降解或分散。
