Rapid synthesis of a 5'-fluorinated oligodeoxy-nucleotide: a model antisense probe for use in imaging with positron emission tomography (PET).

作者信息

Pan D, Gambhir S S, Toyokuni T, Iyer M R, Acharya N, Phelps M E, Barrio J R

机构信息

Crump Institute for Biological Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine 90095-1770, USA.

出版信息

Bioorg Med Chem Lett. 1998 Jun 2;8(11):1317-20. doi: 10.1016/s0960-894x(98)00239-x.

5'-Deoxy-5'-fluoro-O4-methylthymidine was synthesized by the reaction of the corresponding 5'-O-tosylate with KF in the presence of Kryptofix [222] and coupled to a 5'-phosphoramidite-activated CPG-bound oligodeoxynucleotide. The sequence of reactions and purifications were accomplished within 4 h, a necessary condition of the development of radiofluorinated antisense oligodeoxynucleotide probe for use with PET.