Pan D, Gambhir S S, Toyokuni T, Iyer M R, Acharya N, Phelps M E, Barrio J R
Crump Institute for Biological Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine 90095-1770, USA.
Bioorg Med Chem Lett. 1998 Jun 2;8(11):1317-20. doi: 10.1016/s0960-894x(98)00239-x.
5'-Deoxy-5'-fluoro-O4-methylthymidine was synthesized by the reaction of the corresponding 5'-O-tosylate with KF in the presence of Kryptofix [222] and coupled to a 5'-phosphoramidite-activated CPG-bound oligodeoxynucleotide. The sequence of reactions and purifications were accomplished within 4 h, a necessary condition of the development of radiofluorinated antisense oligodeoxynucleotide probe for use with PET.
