von Wintzingerode F, Selent B, Hegemann W, Göbel U B
Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, 10117 Berlin, Germany.
Appl Environ Microbiol. 1999 Jan;65(1):283-6. doi: 10.1128/AEM.65.1.283-286.1999.
A culture-independent phylogenetic survey for an anaerobic trichlorobenzene-transforming microbial community was carried out. Small-subunit rRNA genes were PCR amplified from community DNA by using primers specific for Bacteria or Euryarchaeota and were subsequently cloned. Application of a new hybridization-based screening approach revealed 51 bacterial clone families, one of which was closely related to dechlorinating Dehalobacter species. Several clone sequences clustered to rDNA sequences obtained from a molecular study of an anaerobic aquifer contaminated with hydrocarbons and chlorinated solvents (Dojka et al., Appl. Env. Microbiol. 64:3869-3877, 1998).
对一个厌氧三氯苯转化微生物群落进行了不依赖培养的系统发育调查。使用针对细菌或广古菌的特异性引物,从群落DNA中PCR扩增小亚基rRNA基因,随后进行克隆。一种基于杂交的新筛选方法的应用揭示了51个细菌克隆家族,其中一个与脱氯的脱卤杆菌属物种密切相关。几个克隆序列聚类到从一个被碳氢化合物和氯化溶剂污染的厌氧含水层的分子研究中获得的rDNA序列(Dojka等人,《应用与环境微生物学》64:3869 - 3877,1998年)。