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本文引用的文献

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Rebuttal to correspondence on: Chlorobenzenes in sediments, water and selected fish from Lakes Superior, Huron, Erie and Ontario.对关于“苏必利尔湖、休伦湖、伊利湖和安大略湖沉积物、水中及部分鱼类体内的氯苯”通信的反驳
Environ Sci Technol. 1983 Aug 1;17(8):505. doi: 10.1021/es00114a014.
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Enrichment and properties of a 1,2,4-trichlorobenzene-dechlorinating methanogenic microbial consortium.1,2,4-三氯苯脱氯产甲烷微生物共混物的富集与特性。
Appl Environ Microbiol. 1997 Apr;63(4):1225-9. doi: 10.1128/aem.63.4.1225-1229.1997.
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Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation.正在进行自然生物修复的受碳氢化合物和氯化溶剂污染的含水层中的微生物多样性。
Appl Environ Microbiol. 1998 Oct;64(10):3869-77. doi: 10.1128/AEM.64.10.3869-3877.1998.
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Dehalobacter restrictus gen. nov. and sp. nov., a strictly anaerobic bacterium that reductively dechlorinates tetra- and trichloroethene in an anaerobic respiration.限制脱卤杆菌属及种,一种严格厌氧细菌,在厌氧呼吸过程中对四氯乙烯和三氯乙烯进行还原性脱氯。
Arch Microbiol. 1998 Apr;169(4):313-21. doi: 10.1007/s002030050577.
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Physiological characterization of a bacterial consortium reductively dechlorinating 1,2,3- and 1,2,4-trichlorobenzene.还原脱氯1,2,3-和1,2,4-三氯苯的细菌联合体的生理特性
Appl Environ Microbiol. 1998 Feb;64(2):496-503. doi: 10.1128/AEM.64.2.496-503.1998.
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Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis.环境样品中微生物多样性的测定:基于PCR的rRNA分析的陷阱
FEMS Microbiol Rev. 1997 Nov;21(3):213-29. doi: 10.1111/j.1574-6976.1997.tb00351.x.
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Novel division level bacterial diversity in a Yellowstone hot spring.黄石温泉中全新的科级细菌多样性
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8
Isolation of an anaerobic bacterium which reductively dechlorinates tetrachloroethene and trichloroethene.一种能对四氯乙烯和三氯乙烯进行还原脱氯的厌氧细菌的分离。
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Diversity of uncultured microorganisms associated with the seagrass Halophila stipulacea estimated by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes.通过对PCR扩增的16S rRNA基因进行限制性片段长度多态性分析来估计与海草针叶藻相关的未培养微生物的多样性。
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一个厌氧的、能转化三氯苯的微生物群落的系统发育分析。

Phylogenetic analysis of an anaerobic, trichlorobenzene-transforming microbial consortium.

作者信息

von Wintzingerode F, Selent B, Hegemann W, Göbel U B

机构信息

Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, 10117 Berlin, Germany.

出版信息

Appl Environ Microbiol. 1999 Jan;65(1):283-6. doi: 10.1128/AEM.65.1.283-286.1999.

DOI:10.1128/AEM.65.1.283-286.1999
PMID:9872791
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC91014/
Abstract

A culture-independent phylogenetic survey for an anaerobic trichlorobenzene-transforming microbial community was carried out. Small-subunit rRNA genes were PCR amplified from community DNA by using primers specific for Bacteria or Euryarchaeota and were subsequently cloned. Application of a new hybridization-based screening approach revealed 51 bacterial clone families, one of which was closely related to dechlorinating Dehalobacter species. Several clone sequences clustered to rDNA sequences obtained from a molecular study of an anaerobic aquifer contaminated with hydrocarbons and chlorinated solvents (Dojka et al., Appl. Env. Microbiol. 64:3869-3877, 1998).

摘要

对一个厌氧三氯苯转化微生物群落进行了不依赖培养的系统发育调查。使用针对细菌或广古菌的特异性引物,从群落DNA中PCR扩增小亚基rRNA基因,随后进行克隆。一种基于杂交的新筛选方法的应用揭示了51个细菌克隆家族,其中一个与脱氯的脱卤杆菌属物种密切相关。几个克隆序列聚类到从一个被碳氢化合物和氯化溶剂污染的厌氧含水层的分子研究中获得的rDNA序列(Dojka等人,《应用与环境微生物学》64:3869 - 3877,1998年)。