Lanciotti R S, Ludwig M L, Rwaguma E B, Lutwama J J, Kram T M, Karabatsos N, Cropp B C, Miller B R
Division of Vector-Borne Infectious Diseases, U.S. Department of Health and Human Services, Fort Collins, Colorado, USA.
Virology. 1998 Dec 5;252(1):258-68. doi: 10.1006/viro.1998.9437.
O'nyong-nyong (ONN) virus is an alphavirus (family Togaviridae, genus Alphavirus) classified in the Semliki Forest virus (SFV) antigenic complex. ONN was initially isolated in northern Uganda in 1959 during the early stages of an explosive arbovirus epidemic in which > 2 million cases were reported. No additional epidemics or human isolations of ONN were reported until 1996, when it was isolated from an epidemic in southern Uganda. We report the complete nucleotide and deduced amino acid sequence of one of these 1996-1997 ONN isolates (SG650) and that of the related alphavirus Igbo Ora virus. The data indicate that the recent ONN virus isolate is closely related to the previously published ONN strain isolated in 1959. In addition, phylogenetic analysis of the sequence data reveals that Igbo Ora virus, previously thought to be a separate virus closely related to ONN and Chikungunya (CHIK), clearly is a strain of ONN. The sequence data also reveal that unlike the published ONN (1959) sequence, all ONN strains from the 1996-1997 epidemic possess a stop codon at the nsp3-nsp4 junction.
奥尼昂-尼昂病毒（ONN）是一种甲病毒（披膜病毒科，甲病毒属），归类于塞米基森林病毒（SFV）抗原复合物。1959年，在乌干达北部一场爆发性虫媒病毒疫情的早期，首次分离出了奥尼昂-尼昂病毒，此次疫情报告的病例超过200万例。直到1996年，才再次报告有奥尼昂-尼昂病毒疫情或从人体分离出该病毒，当时它是从乌干达南部的一场疫情中分离出来的。我们报告了1996 - 1997年这些奥尼昂-尼昂病毒分离株之一（SG650）以及相关甲病毒伊博奥拉病毒的完整核苷酸和推导氨基酸序列。数据表明，最近的奥尼昂-尼昂病毒分离株与1959年分离出的先前公布的奥尼昂-尼昂病毒株密切相关。此外，对序列数据的系统发育分析表明，伊博奥拉病毒以前被认为是一种与奥尼昂-尼昂病毒和基孔肯雅病毒（CHIK）密切相关的独立病毒，显然是奥尼昂-尼昂病毒的一个毒株。序列数据还显示，与已公布的1959年奥尼昂-尼昂病毒序列不同，1996 - 1997年疫情中的所有奥尼昂-尼昂病毒株在非结构蛋白3 - 非结构蛋白4连接处都有一个终止密码子。
