Dodd S J, Williams M, Suhan J P, Williams D S, Koretsky A P, Ho C
Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 USA.
Biophys J. 1999 Jan;76(1 Pt 1):103-9. doi: 10.1016/S0006-3495(99)77182-1.
This study reports the detection of single mammalian cells, specifically T cells (T lymphocytes) labeled with dextran-coated superparamagnetic iron oxide particles, using magnetic resonance microscopy. Size amplification due to sequestration of the superparamagnetic particles in vacuoles enhances contrast in localized areas in high-resolution magnetic resonance imaging. Magnetic resonance images of samples containing differing concentrations of T cells embedded in 3% gelatin show a number of dark regions due to the superparamagnetic iron oxide particles, consistent with the number predicted by transmission electron microscopy. Colabeling of T cell samples with a fluorescent dye leads to strong correlations between magnetic resonance and fluorescence microscopic images, showing the presence of the superparamagnetic iron oxide particles at the cell site. This result lays the foundation for our approach to tracking the movement of a specific cell type in live animals and humans.
本研究报告了利用磁共振显微镜对单个哺乳动物细胞，特别是用葡聚糖包被的超顺磁性氧化铁颗粒标记的T细胞（T淋巴细胞）进行检测的情况。由于超顺磁性颗粒被隔离在液泡中而导致的尺寸放大，增强了高分辨率磁共振成像中局部区域的对比度。含有不同浓度T细胞并嵌入3%明胶中的样本的磁共振图像显示，由于超顺磁性氧化铁颗粒，出现了一些暗区，这与透射电子显微镜预测的数量一致。用荧光染料对T细胞样本进行共标记，导致磁共振图像与荧光显微镜图像之间有很强的相关性，表明超顺磁性氧化铁颗粒存在于细胞部位。这一结果为我们在活体动物和人类中追踪特定细胞类型的运动的方法奠定了基础。
