Molday R S, MacKenzie D
J Immunol Methods. 1982 Aug 13;52(3):353-67. doi: 10.1016/0022-1759(82)90007-2.
Ferromagnetic iron dextran particles were prepared by reacting a mixture of ferrous chloride and ferric chloride with dextran polymers under alkaline conditions. Particles purified by gel filtration chromatography were in the size range of 30-40 nm, had an electron dense core of about 15 nm, were stable against aggregation in physiological buffer, showed little non-specific binding to cells and had a magnetic moment. Protein A from Staphylococcus aureus was covalently coupled to periodate-oxidized ferromagnetic iron-dextran particles. These conjugates were used to indirectly label antigen sites on human red blood cells and thymocytes for visualization by scanning and transmission electron microscopy. Cells labeled with these immunospecific ferromagnetic particles are were quantitatively retained by a simple permanent magnet and could be separated from unlabeled cells. Applications of these novel reagents in the separation of cells, cell membranes and receptors in drug targeting studies are discussed.
通过在碱性条件下使氯化亚铁和氯化铁的混合物与葡聚糖聚合物反应来制备铁磁性葡聚糖铁颗粒。经凝胶过滤色谱法纯化的颗粒尺寸范围为30 - 40纳米，具有约15纳米的电子致密核心，在生理缓冲液中抗聚集稳定，对细胞几乎无非特异性结合且具有磁矩。来自金黄色葡萄球菌的蛋白A共价偶联至经高碘酸盐氧化的铁磁性葡聚糖铁颗粒。这些偶联物用于间接标记人红细胞和胸腺细胞上的抗原位点，以便通过扫描和透射电子显微镜进行可视化观察。用这些免疫特异性铁磁性颗粒标记的细胞可被一块简单的永久磁铁定量保留，并可与未标记的细胞分离。讨论了这些新型试剂在药物靶向研究中的细胞、细胞膜和受体分离方面的应用。
