Bulte J W, Hoekstra Y, Kamman R L, Magin R L, Webb A G, Briggs R W, Go K G, Hulstaert C E, Miltenyi S, The T H
Department of Clinical Immunology, University of Groningen, The Netherlands.
Magn Reson Med. 1992 May;25(1):148-57. doi: 10.1002/mrm.1910250115.
Human lymphocytes were labeled with biotinylated anti-lymphocyte-directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran-magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R1. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.
用人淋巴细胞与生物素化的抗淋巴细胞定向单克隆抗体进行标记，然后将链霉亲和素以及随后的生物素化葡聚糖 - 磁铁矿颗粒与之偶联。这种标记导致在2.0 T时淋巴细胞悬液出现强烈且选择性的负对比增强，这主要是由R2的特异性增加以及R1虽小但显著的特异性增加所引起的。发现R1随场强增加而降低。这里描述的免疫标记程序可用于磁共振成像中靶细胞的选择性信号消耗。
