Shan H, Goldman J, Cunto G, Manuppello J, Chaiken I, Cines D B, Silberstein L E
Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, PA, 19104, USA.
J Autoimmun. 1998 Dec;11(6):651-60. doi: 10.1006/jaut.1998.0242.
The role of the anti-phospholipid antibodies (APLA) and anti-endothelial cell antibodies (AECA) in the pathogenesis of anti-phospholipid syndrome (APS) is unclear. Differences in the reported involvement of APLA may be due, in part, to the polyclonal nature of these antibodies and the use of serum and serum fractions for analysis. To circumvent this issue, we generated monoclonal antibodies (MAB) from three patients with APS and two healthy controls. We then compared the antigen binding patterns and the heavy chain variable region (VH) DNA sequences of the MAB derived from patients with APS to those from healthy controls. The results of this study indicate that APLA and AECA comprise a highly heterogeneous population of antibodies with respect to the antigens they recognize, as well as VH gene usage. MAB derived from patients with APS do not differ from those derived from normal individuals based on either antigen recognition or VH gene usage. These results suggest the importance of additional predisposing factors in the pathogenesis of APS.
抗磷脂抗体（APLA）和抗内皮细胞抗体（AECA）在抗磷脂综合征（APS）发病机制中的作用尚不清楚。报道的APLA参与情况的差异，部分可能归因于这些抗体的多克隆性质以及使用血清和血清组分进行分析。为规避这一问题，我们从三名APS患者和两名健康对照者中制备了单克隆抗体（MAB）。然后，我们比较了源自APS患者的MAB与源自健康对照者的MAB的抗原结合模式和重链可变区（VH）DNA序列。本研究结果表明，就所识别的抗原以及VH基因使用情况而言，APLA和AECA包含高度异质性的抗体群体。源自APS患者的MAB在抗原识别或VH基因使用方面与源自正常个体的MAB并无差异。这些结果提示了其他易感因素在APS发病机制中的重要性。
