Vachet R W, Ray K L, Glish G L
Department of Chemistry, University of North Carolina, Chapel Hill 27599, USA.
J Am Soc Mass Spectrom. 1998 Apr;9(4):341-4. doi: 10.1016/S1044-0305(98)00008-7.
Stored waveform inverse Fourier transform and double resonance techniques have been used in conjunction with a quadrupole ion trap to study the dissociation patterns of peptide ions. These experiments provide insight into the origin of individual product ions in an MS/MS spectrum. Results show for a series of leucine enkephalin analogues with five amino acid residues that the b4 ion is the main product ion through which many other product ions arise. It was also observed that the percentage of the a4 product ions that are formed directly from the protonated molecule (M + H)+ depends on the nature of the fourth amino acid residue. In addition, it was determined that in the peptides studies here lower series b ions (e.g., b3) arise from direct dissociation of higher series b ions (e.g., b4) only about 50% of the time.
存储波形傅里叶逆变换和双共振技术已与四极离子阱结合使用，以研究肽离子的解离模式。这些实验有助于深入了解串联质谱（MS/MS）谱中各个产物离子的来源。结果表明，对于一系列具有五个氨基酸残基的亮氨酸脑啡肽类似物，b4离子是许多其他产物离子产生的主要产物离子。还观察到，直接由质子化分子（M + H）+形成的a4产物离子的百分比取决于第四个氨基酸残基的性质。此外，已确定在此处研究的肽中，较低系列的b离子（例如b3）仅约50%的时间是由较高系列的b离子（例如b4）直接解离产生的。
