新型酵母前体mRNA 3'末端加工信号的基因组检测
Genomic detection of new yeast pre-mRNA 3'-end-processing signals.
作者信息
Graber J H, Cantor C R, Mohr S C, Smith T F
机构信息
Center for Advanced Biotechnology, Boston University, 36 Cummington Street, Boston, MA 02215, USA.
出版信息
Nucleic Acids Res. 1999 Feb 1;27(3):888-94. doi: 10.1093/nar/27.3.888.
Abstract
To investigate Saccharomyces cerevisiae 3'-end-processing signals, a set of 1352 unique pre-mRNA 3'-end-processing sites, corresponding to 861 different genes, was identified by alignment of expressed sequence tag sequences with the complete yeast genome. Nucleotide word frequencies in the vicinity of the cleavage sites were analyzed to reveal the signal element features. In addition to previously recognized processing signals, two previously uncharacterized components of the 3'-end-processing signal sequence were discovered, specifically a predominance of U-rich sequences located on either side of the cleavage site. One of these, the downstream U-rich signal, provides a further link between the 3'-end-processing mechanisms of yeast and higher eukaryotes. Analysis of the complete set of 3'-end-processing sites by means of a discrimination function supports a 'contextual' model in which the sum total effectiveness of the signals in all four elements determines whether or not processing occurs.
摘要
为了研究酿酒酵母3'端加工信号，通过将表达序列标签序列与完整的酵母基因组进行比对，鉴定出了一组1352个独特的前体mRNA 3'端加工位点，这些位点对应于861个不同的基因。分析切割位点附近的核苷酸词频以揭示信号元件特征。除了先前识别的加工信号外，还发现了3'端加工信号序列中两个以前未表征的成分，具体而言，是位于切割位点两侧的富含U的序列占优势。其中之一，即下游富含U的信号，在酵母和高等真核生物的3'端加工机制之间提供了进一步的联系。通过判别函数对所有3'端加工位点进行分析，支持了一种“上下文”模型，即所有四个元件中信号的总有效性决定了加工是否发生。
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