Selser J C, Yeh Y, Baskin R J
Biophys J. 1976 Dec;16(12):1357-71. doi: 10.1016/S0006-3495(76)85780-3.
Light-scattering/intensity autocorrelation measurements of vesicle diffusivity were used to follow the time course of the osmotic response of lobster abdominal sarcoplasmic reticulum vesicles to five lipophobic nonelectrolytes. Steady-state portions of the resulting time traces show these vesicles to be permeable to ethylene glycol and glycerol and impermeable to erythritol, glucose, and sucrose. Using measured values of the hydrodynamic radii of these nonelectrolytes, it is concluded that under passive transport conditions, these vesicles may be thought of as having pores whose radii lie between 3.1 and 3.5 A. In addition, the results presented here indicated that above a certain impermeable nonelectrolyte concentration, vesicles did not respond osmotically even though they had not collapsed. This suggests that at least under the experimental conditions reported here, vesicles behaved as if rigid when their average volume had decreased to about 50% of its original isotonic value.
利用囊泡扩散率的光散射/强度自相关测量来跟踪龙虾腹部肌质网囊泡对五种疏脂性非电解质的渗透反应的时间进程。所得时间轨迹的稳态部分表明，这些囊泡对乙二醇和甘油具有通透性，而对赤藓糖醇、葡萄糖和蔗糖则不通透。利用这些非电解质的流体动力学半径的测量值，可以得出结论，在被动运输条件下，这些囊泡可以被认为具有半径在3.1至3.5埃之间的孔。此外，此处给出的结果表明，在高于一定浓度的不可渗透非电解质时，囊泡即使没有塌陷也不会发生渗透反应。这表明至少在本文报道的实验条件下，当囊泡的平均体积降至其原始等渗值的约50%时，其行为就好像是刚性的。
