Szarfman A, Laranja F S, de Souza W, Galvão Quintao L, Gerecht D, Schmuñis G A
Am J Trop Med Hyg. 1978 Jul;27(4):832-4. doi: 10.4269/ajtmh.1978.27.832.
Immunoglobulins (Igs) reacting against endothelial and vascular structures and striated muscle cells as well as against cells from a peripheral nerve were detected by indirect immunofluorescent test (IIF) in a rhesus monkey infected for 29 yr with Trypanosoma cruzi. Anti-T. cruzi antibodies in this monkey showed a titer of 1:128 in the IIF test and the direct agglutination test. Tissue-reacting Igs were bound in vivo to the tissues, as was established by direct treatment of a biopsy of the deltoid muscle with Ig-labeled antisera. Electron microscopy of this tissue showed that Igs reacted with the plasma membrane of the muscle cells. Neither tissue-reacting Igs nor specific antibodies were detected in three uninfected adult monkeys.
在一只感染克氏锥虫29年的恒河猴中，通过间接免疫荧光试验（IIF）检测到了与内皮和血管结构、横纹肌细胞以及外周神经细胞发生反应的免疫球蛋白（Ig）。在IIF试验和直接凝集试验中，这只猴子的抗克氏锥虫抗体滴度为1:128。组织反应性Ig在体内与组织结合，这是通过用Ig标记的抗血清直接处理三角肌活检组织来确定的。该组织的电子显微镜检查表明，Ig与肌肉细胞的质膜发生反应。在三只未感染的成年猴子中未检测到组织反应性Ig和特异性抗体。
