Bunney B S, Aghajanian G K
Brain Res. 1976 Dec 3;117(3):423-35. doi: 10.1016/0006-8993(76)90751-4.
Afferent innervation of the rat substantia nigra (SN) was studied by the retrograde horseradish peroxidase (HRP) method. High concentrations of HRP were deposited in discrete subregions of the SN by means of a microiontophoretic delivery system. Using this technique it was possible to demonstrate that the caudatonigral projection system is arranged topographically; All portions of the caudate-putamen except for a central medial core were found to contain HRP positive cells, indicative of retrograde transport. In the positive areas a much larger percentage of cells (30-50%) were found to participate in this projection than has previously been reported. Only medium size cells (12-20 mum) were found to contain the HRP reaction product. Other areas found to heavily innervate the SN were the globus pallidus, central nucleus of the amygdala and dorsal raphe nucleus. Areas containing fewer reactive cells but which also appear to innervate the SN included the prefrontal cortex and lateral habenula. These results emphasize the importance of striatonigral projections which recent studies have suggested contain a GABAergic link.
采用逆行辣根过氧化物酶（HRP）法研究了大鼠黑质（SN）的传入神经支配。通过微离子电泳给药系统将高浓度的HRP注入SN的不同亚区域。利用该技术可以证明尾状核 - 黑质投射系统是按拓扑结构排列的；除中央内侧核心外，尾状核 - 壳核的所有部分均发现含有HRP阳性细胞，表明存在逆行运输。在阳性区域中，发现参与该投射的细胞百分比（30 - 50%）比以前报道的要高得多。仅发现中等大小的细胞（12 - 20μm）含有HRP反应产物。其他发现大量支配SN的区域有苍白球、杏仁核中央核和中缝背核。含有较少反应性细胞但似乎也支配SN的区域包括前额叶皮质和外侧缰核。这些结果强调了纹状体 - 黑质投射的重要性，最近的研究表明该投射包含一个GABA能连接。
