从梨形四膜虫大核制备含核糖体脱氧核糖核酸的染色质
Preparation of chromatin containing ribosomal deoxyribonucleic acid from the macronucleus of Tetrahymena pyriformis.
作者信息
Jones R W
出版信息
Biochem J. 1978 Jul 1;173(1):145-53. doi: 10.1042/bj1730145.
Abstract
A method is described that enables a chromatin fraction containing ribosomal DNA (DNA containing sequences coding for rRNA) to be prepared from the macronuclei of growing or stationary cultures of Tetrahymena pyriformis. This material is obtained in yields of between 25 and 75% of the theoretical maximum. The DNA in this fraction was identified as ribosomal DNA on the basis of its density and molecular weight, and it appears not to be appreciably contaminated by other DNA. The method relies on the approximate assumption that ribosomal DNA is the smallest species of DNA in chromatin in the nucleus, and avoids the use of mechanical force, or enzyme action, to fractionate chromatin.
摘要
本文描述了一种方法，该方法能够从梨形四膜虫生长或静止培养物的大核中制备出含有核糖体DNA（编码rRNA的DNA序列）的染色质组分。这种材料的产量为理论最大值的25%至75%。该组分中的DNA根据其密度和分子量被鉴定为核糖体DNA，并且似乎没有明显受到其他DNA的污染。该方法基于核糖体DNA是细胞核染色质中最小的DNA种类这一近似假设，并且避免使用机械力或酶作用来分离染色质。
相似文献
1
Preparation of chromatin containing ribosomal deoxyribonucleic acid from the macronucleus of Tetrahymena pyriformis.从梨形四膜虫大核制备含核糖体脱氧核糖核酸的染色质
Biochem J. 1978 Jul 1;173(1):145-53. doi: 10.1042/bj1730145.
2
Histone composition of a chromatin fraction containing ribosomal deoxyribonucleic acid isolated from the macronucleus of Tetrahymena pyriformis.从梨形四膜虫大核中分离出的含有核糖体脱氧核糖核酸的染色质组分的组蛋白组成。
Biochem J. 1978 Jul 1;173(1):155-64. doi: 10.1042/bj1730155.
3
Autonomous rDNA molecules containing single copies of the ribosomal RNA genes in the macronucleus of Tetrahymena pyriformis.含有梨形四膜虫大核中核糖体RNA基因单拷贝的自主rDNA分子。
Biochem Biophys Res Commun. 1974 Aug 19;59(4):1356-65. doi: 10.1016/0006-291x(74)90463-x.
4
The DNA of Tetrahymena pyriformis GL strain. A mild method for preparation and its characterization.梨形四膜虫GL株的DNA。一种温和的制备方法及其特性
Biochim Biophys Acta. 1973 Apr 11;299(4):507-15. doi: 10.1016/0005-2787(73)90222-0.
5
Induction of nucleolar and mitochondrial DNA replication in Tetrahymena pyriformis.梨形四膜虫核仁与线粒体DNA复制的诱导
Proc Natl Acad Sci U S A. 1974 Mar;71(3):894-8. doi: 10.1073/pnas.71.3.894.
6
Histone F1 of Tetrahymena macronuclei: unique electrophoretic properties and phosphorylation of F1 in an amitotic nucleus.嗜热四膜虫大核组蛋白F1：独特的电泳性质及无丝分裂细胞核中F1的磷酸化作用
J Cell Biol. 1974 Apr;61(1):134-45. doi: 10.1083/jcb.61.1.134.
7
A single integrated gene for ribosomal RNA in a eucaryote, Tetrahymena pyriformis.梨形四膜虫（一种真核生物）中核糖体RNA的单一整合基因。
Cell. 1977 Sep;12(1):121-32. doi: 10.1016/0092-8674(77)90190-8.
8
[Distribution of repeating nucleotide sequences in the macronucleus DNA of Tetrahymena infusoria].[四膜虫大核DNA中重复核苷酸序列的分布]
Mol Biol (Mosk). 1978 Jul-Aug;12(4):894-900.
9
The histone content of Tetrahymena ribosomal gene-containing chromatin.含有四膜虫核糖体基因的染色质的组蛋白含量。
J Biol Chem. 1983 May 10;258(9):5944-54.
10
DNase I sensitivity of ribosomal genes in isolated nucleosome core particles.核糖体基因在分离的核小体核心颗粒中的脱氧核糖核酸酶I敏感性
Nucleic Acids Res. 1980 Jan 11;8(1):197-214. doi: 10.1093/nar/8.1.197-e.
引用本文的文献
1
Applications of Model-Based Meta-Analysis in Drug Development.基于模型的荟萃分析在药物研发中的应用。
Pharm Res. 2022 Aug;39(8):1761-1777. doi: 10.1007/s11095-022-03201-5. Epub 2022 Feb 16.
2
Non-nucleosomal packaging of a tandemly repeated DNA sequence at termini of extrachromosomal DNA coding for rRNA in Tetrahymena.嗜热四膜虫染色体外DNA编码rRNA末端串联重复DNA序列的非核小体包装。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2263-7. doi: 10.1073/pnas.78.4.2263.
3
Transcriptional properties of nucleoli isolated from Tetrahymena.从四膜虫中分离出的核仁的转录特性。
Nucleic Acids Res. 1978 Nov;5(11):3993-4006. doi: 10.1093/nar/5.11.3993.
4
Histone composition of a chromatin fraction containing ribosomal deoxyribonucleic acid isolated from the macronucleus of Tetrahymena pyriformis.从梨形四膜虫大核中分离出的含有核糖体脱氧核糖核酸的染色质组分的组蛋白组成。
Biochem J. 1978 Jul 1;173(1):155-64. doi: 10.1042/bj1730155.
本文引用的文献
1
Preparation of native chromatin and damage caused by shearing.制备天然染色质和剪切引起的损伤。
Science. 1975 Mar 28;187(4182):1203-6. doi: 10.1126/science.187.4182.1203.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Studies on nuclear structure and function in Tetrahymena pyriformis. II. Isolation of macro- and micronuclei.梨形四膜虫细胞核结构与功能的研究。II. 大核和小核的分离。
J Cell Biol. 1970 Dec;47(3):619-30. doi: 10.1083/jcb.47.3.619.
4
Synchronization of the cell cycle of Tetrahymena by stravation and refeeding.通过饥饿和再投喂使四膜虫的细胞周期同步化。
J Protozool. 1970 Aug;17(3):429-31. doi: 10.1111/j.1550-7408.1970.tb04708.x.
5
Nucleolar organization and ribosome formation in Tetrahymena pyriformis GL.梨形四膜虫GL中的核仁组织与核糖体形成
Exp Cell Res. 1970 Jun;60(3):361-72. doi: 10.1016/0014-4827(70)90529-x.
6
Free ribosomal RNA genes in the macronucleus of Tetrahymena.四膜虫大核中的游离核糖体RNA基因。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3078-81. doi: 10.1073/pnas.71.8.3078.
7
Comparison of the sequences of macro- and micronuclear DNA of Tetrahymena pyriformis.梨形四膜虫大核和小核DNA序列的比较。
Chromosoma. 1974;48(1):1-18. doi: 10.1007/BF00284863.
8
Autonomous rDNA molecules containing single copies of the ribosomal RNA genes in the macronucleus of Tetrahymena pyriformis.含有梨形四膜虫大核中核糖体RNA基因单拷贝的自主rDNA分子。
Biochem Biophys Res Commun. 1974 Aug 19;59(4):1356-65. doi: 10.1016/0006-291x(74)90463-x.
9
The use of non-ionic industrial detergents in liquid scintillation counting.非离子工业洗涤剂在液体闪烁计数中的应用。
Lab Pract. 1975 Nov;24(11):739-40.
10
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.用EcoRI限制性内切酶切割噬菌体λ DNA的研究。
J Mol Biol. 1975 Jan 25;91(3):315-28. doi: 10.1016/0022-2836(75)90383-6.
Zotero 插件