Ochi Y, Hachiya T, Yoshimura M, Miyazaki T, Majima T, Takahashi H
Endocrinol Jpn. 1976 Jun;23(3):207-13. doi: 10.1507/endocrj1954.23.207.
This study was undertaken to determine T3 content in red cells by radioimmunoassay. T3 in red blood cells was solubilized fairly from the stroma by hemolysis and red-cell T3 content could be determined directly by radioimmunoassay of the lysate. After hemolysing red cells with an equal volume of distilled water, 0.4 ml of the hemolyzate was used for the assay. The red-cell T3 content was expressed as ng/ml of red-cell volume. The normal T3 range in red cells was 0.20-0.45 ng/ml, and the Mean+/-SD was 0.32+/-0.10 ng/ml. The limit of detectability was 0.2 ng/ml. In hyperthyroid patients, the red-cell T3 content was more than 0.50 ng/ml with a Mean +/-SD of 1.35+/-0.65 ng/ml. In hypothyroid patients, red cells contained less than 0.25 ng/ml of T3, and there was an overlap from 0.20 to 0.25 ng/ml in the content of red-cell T3 in hypothyroid and euthyroid subjects. The patients with T3 toxicosis showed a high or normal level of red-cell T3. A positive correlation was noted between the red-cell T3 content and the serum T3 level (r=0.66). The correlation between the red-cell T3 content and the free T4 index (expressed as T7) was also positive (r=0.67). From these experiments, it is suggested that the red-cell T3 is low in comparison with the serum T3 levels, and depends on two factors; serum T4 and serum T3 levels.
本研究旨在通过放射免疫分析法测定红细胞中的T3含量。红细胞中的T3通过溶血能较好地从基质中溶解出来，红细胞T3含量可通过对裂解液进行放射免疫分析直接测定。用等体积蒸馏水使红细胞溶血后，取0.4 ml溶血产物用于分析。红细胞T3含量以每毫升红细胞体积中的纳克数表示。红细胞中T3的正常范围是0.20 - 0.45 ng/ml，平均值±标准差为0.32±0.10 ng/ml。可检测极限为0.2 ng/ml。甲状腺功能亢进患者的红细胞T3含量超过0.50 ng/ml，平均值±标准差为1.35±0.65 ng/ml。甲状腺功能减退患者的红细胞中T3含量低于0.25 ng/ml，甲状腺功能减退和甲状腺功能正常的受试者红细胞T3含量在0.20至0.25 ng/ml之间存在重叠。T3中毒患者的红细胞T3水平高或正常。红细胞T3含量与血清T3水平之间呈正相关（r = 0.66）。红细胞T3含量与游离T4指数（以T7表示）之间的相关性也呈阳性（r = 0.67）。从这些实验可以看出，与血清T3水平相比，红细胞T3含量较低，且取决于两个因素：血清T4和血清T3水平。
