Plotkin G M, Brigham S C, Wolf G, Jacobs J B, Arai M
Cancer Biochem Biophys. 1977;2(2):59-63.
Urine samples of normal male Fischer rats or rats fed 0.2% N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide for 6,8 or 30 weeks were collected and centrifuged 50 weeks after beginning treatment. After being sonicated and assayed (with purified desialylated ovine submaxillary mucin as acceptor glycoprotein), the exfoliated bladder cells obtained from the urines of treated rats showed uridine 5'-diphosphate galactose:glycoprotein transferase activity. The specific enzymatic activity of the enzyme from cells of 30-week-treated rats was about 10 times higher than from normal rats. The enzyme from cells of hyperplastic rats (treated 6 or 8 weeks) was only slightly higher in specific activity than that of normal rats. A similar was obtained at a later stage of bladder tumor induction, when the urines from 30-week-treated rats contained blood. A correction was made for protein contributed by the blood clot. The possibility that the blood clot contributed galactosyl transferase activity was excluded. Activity of the enzyme was detected in normal rat bladder tissue and in normal human urine.
收集正常雄性Fischer大鼠或喂食0.2% N-[4-(5-硝基-2-呋喃基)-2-噻唑基]甲酰胺6周、8周或30周的大鼠的尿液样本，并在开始治疗50周后进行离心。对处理过的大鼠尿液中获得的脱落膀胱细胞进行超声处理和检测（以纯化的去唾液酸羊颌下粘蛋白作为受体糖蛋白）后，显示出尿苷5'-二磷酸半乳糖：糖蛋白转移酶活性。处理30周的大鼠细胞中的该酶的比酶活性比正常大鼠高约10倍。增生大鼠（处理6周或8周）细胞中的酶的比活性仅略高于正常大鼠。在膀胱肿瘤诱导后期也得到了类似结果，此时处理30周的大鼠尿液中含有血液。对血凝块贡献的蛋白质进行了校正。排除了血凝块贡献半乳糖基转移酶活性的可能性。在正常大鼠膀胱组织和正常人尿液中检测到了该酶的活性。
