Wattavidanage J, Carter R, Perera K L, Munasingha A, Bandara S, McGuinness D, Wickramasinghe A R, Alles H K, Mendis K N, Premawansa S
Malaria Research Unit, Department of Parasitology, Faculty of Medicine, Colombo, Sri Lanka.
Clin Exp Immunol. 1999 Feb;115(2):350-5. doi: 10.1046/j.1365-2249.1999.00804.x.
We have investigated the association between alleles of the genes for tumour necrosis factor-alpha (TNF-alpha) and TNF-beta and severity of disease during malarial (Plasmodium falciparum) and other infections in the Sri Lankan population. Patients were categorized as having either (i) uncomplicated malaria, (ii) severe and complicated malaria, or (iii) severe and complicated infection in which a diagnosis of malaria had been excluded. For all the patients, as well as for a group of matched healthy controls, TNF-alpha and TNF-beta allelic types were identified using the polymerase chain reaction (PCR) and allele-specific oligonucleotide probes and restriction enzyme digestion. The odds in favour of carrying the TNFalpha2 allele, mainly of the heterozygous genotype (TNFalpha1,*2), were two to three times greater among individuals with severe disease, of either malarial or other infectious origin, relative to healthy controls or to those with uncomplicated malarial infections. No significant risk was associated with either of the alleles of TNF-beta.
我们研究了肿瘤坏死因子-α（TNF-α）和TNF-β基因的等位基因与斯里兰卡人群疟疾（恶性疟原虫）及其他感染期间疾病严重程度之间的关联。患者被分为以下几类：（i）非复杂性疟疾，（ii）严重和复杂性疟疾，或（iii）已排除疟疾诊断的严重和复杂性感染。对于所有患者以及一组匹配的健康对照，使用聚合酶链反应（PCR）、等位基因特异性寡核苷酸探针和限制性酶切来鉴定TNF-α和TNF-β等位基因类型。相对于健康对照或非复杂性疟疾感染患者，患有严重疾病（无论是疟疾还是其他感染源）的个体携带TNFα2等位基因（主要是杂合基因型TNFα1,*2）的几率要高出两到三倍。TNF-β的任何一个等位基因均未显示出显著风险。
