Lainson F A, Aitchison K D, Donachie W, Thomson J R
Moredun Research Institute, Pentland Science Park, Bush Loan, Edinburgh, EH26 0PZ, Scotland.
J Clin Microbiol. 2002 Feb;40(2):588-93. doi: 10.1128/JCM.40.2.588-593.2002.
Porcine dermatitis and nephropathy syndrome (PDNS) is a sporadic, usually fatal disease of growing and finishing pigs that has been recognized in many pig-producing countries. Pasteurella multocida strains isolated from 15 pigs with PDNS and 51 pigs without PDNS were characterized by capsule and somatic antigen typing, random amplified polymorphic DNA (RAP-D) typing, and restriction analysis of genomic DNA using pulsed-field gel electrophoresis (PFGE). While capsular, somatic, and RAP-D typing did not discriminate PDNS isolates from non-PDNS isolates, all of the isolates from PDNS cases showed an identical ApaI PFGE restriction pattern. This pattern was also found in a high proportion (36%) of P. multocida strains isolated from non-PDNS cases. Isolation of a single variant of P. multocida from tissues of pigs with PDNS warrants further investigation into the possible role of these bacteria in the etiology of the disease.
猪皮炎和肾病综合征（PDNS）是一种在生长育肥猪中散发的、通常致命的疾病，已在许多养猪国家被发现。从15头患有PDNS的猪和51头未患PDNS的猪中分离出的多杀性巴氏杆菌菌株，通过荚膜和菌体抗原分型、随机扩增多态性DNA（RAP-D）分型以及使用脉冲场凝胶电泳（PFGE）对基因组DNA进行限制性分析来进行鉴定。虽然荚膜、菌体和RAP-D分型无法区分PDNS分离株和非PDNS分离株，但所有来自PDNS病例的分离株都显示出相同的ApaI PFGE限制性图谱。在从非PDNS病例中分离出的多杀性巴氏杆菌菌株中，也有很大比例（36%）呈现出这种图谱。从患有PDNS的猪组织中分离出单一变种的多杀性巴氏杆菌，这使得有必要进一步研究这些细菌在该疾病病因学中可能扮演的角色。
