Hovius K E, Rijpkema S G, Westers P, van der Zeijst B A, van Asten F J, Houwers D J
Companion Animal Hospital 't Heike, Veldhoven, The Netherlands.
Vet Q. 1999 Jan;21(1):16-20. doi: 10.1080/01652176.1999.9694985.
Thirty-three family dogs were monitored for antibodies to Borrelia burgdorferi sensu lato over a 3-year period. Serum samples were collected before and during the season of high tick activity. Antibody levels were measured with an ELISA based on whole-cell antigens and an ELISA with a purified recombinant flagellin (r410). Antibody levels measured with the whole-cell ELISA increased after the first exposure to ticks. Following the first seasonal period of tick quiescence, antibody levels decreased, and subsequently increased again in the second tick season. Thereafter whole-cell ELISA titres persisted at moderate levels and did not decrease between tick seasons. The recombinant flagellin ELISA did not show a strong response in the first tick season, but did in the second tick season and levels of antibodies continued to fluctuate thereafter. We conclude that most dogs in this study developed an antibody response against Borrelia burgdorferi sensu lato after their first tick infestation and were thereafter repeatedly immunologically stimulated, probably reinfected, during the consecutive tick seasons.
在3年的时间里，对33只家养犬进行了莱姆病疏螺旋体广义种抗体监测。在蜱虫活动频繁的季节之前和期间采集血清样本。使用基于全细胞抗原的酶联免疫吸附测定（ELISA）和含有纯化重组鞭毛蛋白（r410）的ELISA来测量抗体水平。用全细胞ELISA测量的抗体水平在首次接触蜱虫后升高。在蜱虫首次静止的季节之后，抗体水平下降，随后在第二个蜱虫季节再次升高。此后，全细胞ELISA滴度维持在中等水平，在蜱虫季节之间没有下降。重组鞭毛蛋白ELISA在第一个蜱虫季节没有显示出强烈反应，但在第二个蜱虫季节有反应，此后抗体水平继续波动。我们得出结论，本研究中的大多数犬在首次感染蜱虫后产生了针对莱姆病疏螺旋体广义种的抗体反应，此后在连续的蜱虫季节中反复受到免疫刺激，可能再次感染。
