Morriss D M, Lawson J W, Rogolsky M
Antimicrob Agents Chemother. 1978 Aug;14(2):218-23. doi: 10.1128/AAC.14.2.218.
Phage group 2 staphylococcal strain UT0002 contains a large 56S virulence plasmid with genes that code for both exfoliative toxin and a specific staphylococcin termed Bac R(1). Four penicillinase-producing strains and three penicillin-susceptible strains of Neisseria gonorrhoeae were killed by Bac R(1). After 30 min of growth of the penicillin-resistant TR1 strain in 62.5 arbitrary units of Bac R(1) per ml, loss of viability was approximately 90%, and, after 5 h, an approximately 99.99% loss of viability was observed. Lysis did not accompany cell death, and 84% of the Bac R(1) added to the growth medium was adsorbed to the gonococcal cells. The extracellular supernatant fluid from a substrain of staphylococcal strain UT0002 cured of the plasmid for Bac R(1) production had no lethal effect on the gonococcal strains. Bac R(1) was also shown to have bactericidal activity against an L-form of N. meningitidis, indicating that the outer envelope of a neisserial cell is not needed for bacteriocin activity. Ten different normal human sera were unable to neutralize Bac R(1) activity. The bacteriocin lacks adsorption specificity. It binds to but does not kill Escherichia coli cells, indicating that the cell envelope of gram-negative organisms can provide protection against the staphylococcin.
噬菌体2组葡萄球菌菌株UT0002含有一个大型56S毒力质粒，其上的基因编码剥脱毒素和一种名为Bac R(1)的特定葡萄球菌素。四种产青霉素酶的淋病奈瑟菌菌株和三种对青霉素敏感的淋病奈瑟菌菌株被Bac R(1)杀死。青霉素耐药的TR1菌株在每毫升62.5个任意单位的Bac R(1)中生长30分钟后，存活率损失约90%，5小时后，存活率损失约99.99%。细胞死亡时不伴有裂解，添加到生长培养基中的Bac R(1)有84%吸附到淋球菌细胞上。葡萄球菌菌株UT0002的一个亚菌株，其用于产生Bac R(1)的质粒已被消除，该亚菌株的细胞外上清液对淋球菌菌株没有致死作用。Bac R(1)还显示出对脑膜炎奈瑟菌L型有杀菌活性，这表明奈瑟菌细胞的外膜对于细菌素活性不是必需的。十种不同的正常人血清无法中和Bac R(1)的活性。这种细菌素缺乏吸附特异性。它能结合大肠杆菌细胞但不将其杀死，这表明革兰氏阴性菌的细胞膜可以提供对葡萄球菌素的保护。
