Guettler M V, Rumler D, Jain M K
MBI International, Lansing, MI 48909, USA.
Int J Syst Bacteriol. 1999 Jan;49 Pt 1:207-16. doi: 10.1099/00207713-49-1-207.
Strain 130ZT was isolated from the bovine rumen. It is a facultatively anaerobic, pleomorphic, Gram-negative rod. It exhibits a 'Morse code' form of morphology, which is characteristic of the genus Actinobacillus. Strain 130ZT is a capnophilic, osmotolerant succinogen that utilizes a broad range of sugars. It accumulates high concentrations of succinic acid (> 70 g l-1). Strain 130ZT is positive for catalase, oxidase, alkaline phosphatase and beta-galactosidase, but does not produce indole or urease. Acid but no gas is produced from D-glucose and D-fructose. 16S rRNA sequence analysis places strain 130ZT within the family Pasteurellaceae; the most closely related members of the family Pasteurellaceae have 16S rRNA similarities of 95.5% or less with strain 130ZT. Strain 130ZT was compared with Actinobacillus lignieresii and the related Bisgaard Taxa 6 and 10. Based upon morphological and biochemical properties, strain 130ZT is most similar to members of the genus Actinobacillus within the family Pasteurellaceae. It is proposed that strain 130ZT be classified as a new species, Actinobacillus succinogenes. The type strain of Actinobacillus succinogenes sp. nov. is ATCC 55618T.
130ZT菌株是从牛瘤胃中分离出来的。它是兼性厌氧、多形性、革兰氏阴性杆菌。它呈现出“莫尔斯电码”形式的形态，这是放线杆菌属的特征。130ZT菌株是嗜二氧化碳、耐渗透压的琥珀酸产生菌，能利用多种糖类。它积累高浓度的琥珀酸（>70 g l-1）。130ZT菌株过氧化氢酶、氧化酶、碱性磷酸酶和β-半乳糖苷酶呈阳性，但不产生吲哚或脲酶。D-葡萄糖和D-果糖产酸但不产气。16S rRNA序列分析将130ZT菌株归入巴斯德氏菌科；巴斯德氏菌科中关系最密切的成员与130ZT菌株的16S rRNA相似性为95.5%或更低。将130ZT菌株与林氏放线杆菌以及相关的比斯加德分类群6和10进行了比较。基于形态和生化特性，130ZT菌株与巴斯德氏菌科内放线杆菌属的成员最相似。建议将130ZT菌株分类为一个新物种，琥珀酸放线杆菌。琥珀酸放线杆菌新种的模式菌株是ATCC 55618T。
