Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Switzerland.
BASF SE, Ludwigshafen, Germany.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):44-50. doi: 10.1099/ijs.0.011809-0. Epub 2009 Jul 31.
Gram-negative, coccoid, non-motile bacteria that are catalase-, urease- and indole-negative, facultatively anaerobic and oxidase-positive were isolated from the bovine rumen using an improved selective medium for members of the Pasteurellaceae. All strains produced significant amounts of succinic acid under anaerobic conditions with glucose as substrate. Phenotypic characterization and multilocus sequence analysis (MLSA) using 16S rRNA, rpoB, infB and recN genes were performed on seven independent isolates. All four genes showed high sequence similarity to their counterparts in the genome sequence of the patent strain MBEL55E, but less than 95 % 16S rRNA gene sequence similarity to any other species of the Pasteurellaceae. Genetically these strains form a very homogeneous group in individual as well as combined phylogenetic trees, clearly separated from other genera of the family from which they can also be separated based on phenotypic markers. Genome relatedness as deduced from the recN gene showed high interspecies similarities, but again low similarity to any of the established genera of the family. No toxicity towards bovine, human or fish cells was observed and no RTX toxin genes were detected in members of the new taxon. Based on phylogenetic clustering in the MLSA analysis, the low genetic similarity to other genera and the phenotypic distinction, we suggest to classify these bovine rumen isolates as Basfia succiniciproducens gen. nov., sp. nov. The type strain is JF4016(T) (=DSM 22022(T) =CCUG 57335(T)).
从牛瘤胃中使用改良的巴斯德氏菌选择性培养基分离出革兰氏阴性、球形、非运动、过氧化氢酶-、脲酶-和吲哚阴性、兼性厌氧和氧化酶阳性的细菌。所有菌株在以葡萄糖为底物的厌氧条件下都能产生大量琥珀酸。对 7 个独立分离株进行了表型特征分析和多位点序列分析（MLSA），使用 16S rRNA、rpoB、infB 和 recN 基因。这四个基因与专利菌株 MBEL55E 的基因组序列中的对应基因具有高度的序列相似性，但与巴斯德氏菌科的任何其他物种的 16S rRNA 基因序列相似性都不到 95%。这些菌株在个体和组合系统发育树中形成了一个非常同质的群体，与家族中的其他属明显分开，也可以根据表型标记与其他属分开。从 recN 基因推断的基因组相关性显示出高度的种间相似性，但与该科任何已建立的属的相似性仍然很低。未观察到对牛、人或鱼细胞的毒性，也未在新分类群的成员中检测到 RTX 毒素基因。基于 MLSA 分析中的系统发育聚类、与其他属的低遗传相似性和表型差异，我们建议将这些牛瘤胃分离株分类为巴斯夫氏菌属（Basfia） succiniciproducens 属，种，新型。模式株为 JF4016（T）（=DSM 22022（T）=CCUG 57335（T））。
