一种来自金黄色葡萄球菌的限制性内切酶。
A restriction endonuclease from Staphylococcus aureus.
作者信息
Sussenbach J S, Monfoort C H, Schiphof R, Stobberingh E E
出版信息
Nucleic Acids Res. 1976 Nov;3(11):3193-202. doi: 10.1093/nar/3.11.3193.
A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded phi X174 DNA. It recognizes the sequence (see article) and cleaves as indicated by the arrows. Evidence is presented that this enzyme plays a role in the biological restriction-modification system of Staphylococcus aureus strain 3A.
一种特定的核酸内切酶Sau 3AI已通过DEAE-纤维素色谱法从金黄色葡萄球菌3A菌株中部分纯化出来。该酶能多次切割5型腺病毒DNA，切割SV40 DNA八次，但不切割双链φX174 DNA。它识别特定序列（见文章）并按箭头所示进行切割。有证据表明，这种酶在金黄色葡萄球菌3A菌株的生物限制修饰系统中发挥作用。
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