Shraideh Z A, Abu-Elteen K H, Sallal A K
Department of Biological Sciences, University of Jordan, Amman.
Mycopathologia. 1998;142(3):119-23. doi: 10.1023/a:1006901019786.
The effect of Berhi date extract on the ultrastructure of Candida albicans was studied by scanning and transmission electron microscopy. Exposure of yeast to 5% (w/v) date extract showed evidence of weakening in the cell wall with indications of cell distortion and partial collapse in some cases as seen by scanning electron microscopy. Increasing the concentration of date extract (20%, w/v) led to more drastic damage to the yeast with cell lysis and concurrent leakage of cytoplasmic material with eventual cell death. Ultrastructural investigation showed irregular shapes of cells treated with date extract, with prominent effects on cell wall layers. Cell membranes lost their integrity, aggregation of the cytoplasmic contents and large detachment of plasmalemma from cell wall was observed in the treated cells. These results suggest that date extract may have multiple effects on Candida with an increasing potential of using it for prophylaxis purposes.
通过扫描电子显微镜和透射电子显微镜研究了椰枣提取物对白色念珠菌超微结构的影响。扫描电子显微镜观察发现，将酵母暴露于5%（w/v）的枣提取物中时，细胞壁有减弱的迹象，在某些情况下有细胞变形和部分塌陷的迹象。增加枣提取物的浓度（20%，w/v）会对酵母造成更严重的损害，导致细胞裂解，同时细胞质物质泄漏，最终细胞死亡。超微结构研究显示，用枣提取物处理的细胞形状不规则，对细胞壁层有显著影响。在处理过的细胞中观察到细胞膜失去完整性、细胞质内容物聚集以及质膜与细胞壁大量分离。这些结果表明，枣提取物可能对念珠菌有多种作用，并且越来越有用于预防目的的潜力。
