一种用于在多种临床标本中检测结核分枝杆菌的快速聚合酶链反应技术。
A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens.
作者信息
Kearns A M, Freeman R, Steward M, Magee J G
机构信息
Public Health Laboratory, General Hospital, Newcastle upon Tyne, UK.
出版信息
J Clin Pathol. 1998 Dec;51(12):922-4. doi: 10.1136/jcp.51.12.922.
Abstract
A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical material. Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M tuberculosis in 100% of smear positive, culture positive samples. The in-house assay proved to be rapid, easy, and inexpensive to perform, and the inclusion of an internal inhibitor control permitted validation of the PCR results.
摘要
本文描述了一种用于直接检测临床样本中结核分枝杆菌复合群的快速内部聚合酶链反应（PCR）检测方法。将其性能与两种基于试剂盒的系统进行了比较。内部检测结果与商业检测相当，在100%的涂片阳性、培养阳性样本中检测到结核分枝杆菌。内部检测方法被证明快速、简便且成本低廉，并且包含内部抑制剂对照可验证PCR结果。
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